Regulation of immune responses by T suppressor cells and by serum in chronic paracoccidioidomycosis

Elizabeth Castaneda, Elmer Brummer, Demosthenes Pappagianis, David A. Stevens

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Regulation of cellular responses was studied during the course of chronic murine disseminated paracoccidioidomycosis. Regulation of peripheral blood lymphocyte (PBL) proliferative responses to concanavalin A (Con A) was studied in vitro by mixing PBL from infected and noninfected mice. PBL from mice infected for 18 weeks had depressed responses to Con A and they depressed the Con A responses of PBL from noninfected mice by 95% when they were mixed in a 1:1 ratio. After treatment of PBL from infected mice with anti-Lyt-2.2 antibody plus complement, the responses to Con A were increased to normal values. The percentage of T-cell subpopulations in PBL from infected mice did not differ significantly from those of normal mice. Immunoregulation of delayed-type hypersensitivity (DTH) responses to antigen by serum from infected animals was studied in mice 1 week after intranasal (i.n.) infection, a time when DTH responses were maximal. DTH responses to antigen 7 days after i.n. infection (107 CFU Paracoccidioides brasiliensis) were significantly reduced when 0.5 ml of immune mouse serum (ELISA antibody titer to P. brasiliensis antigens 1:10,240) was given i.v. 1 day before infection (P < 0.01) or 1 day before skin testing (P < 0.001). Normal mouse serum did not have this effect. The results indicate that progression of chronic disseminated paracoccidioidomycosis was associated with the development of T-cell suppressor activity for Con A responses of PBL, and that DTH responses to antigen were depressed by the administration of serum with specific high titer antibodies.

Original languageEnglish (US)
Pages (from-to)1-11
Number of pages11
JournalCellular Immunology
Volume117
Issue number1
DOIs
StatePublished - 1988

Fingerprint

Paracoccidioidomycosis
Concanavalin A
Lymphocytes
Serum
Delayed Hypersensitivity
Paracoccidioides
Antigens
Infection
T-Lymphocytes
Antibodies
Immune Sera
Reference Values
Enzyme-Linked Immunosorbent Assay

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Regulation of immune responses by T suppressor cells and by serum in chronic paracoccidioidomycosis. / Castaneda, Elizabeth; Brummer, Elmer; Pappagianis, Demosthenes; Stevens, David A.

In: Cellular Immunology, Vol. 117, No. 1, 1988, p. 1-11.

Research output: Contribution to journalArticle

Castaneda, Elizabeth ; Brummer, Elmer ; Pappagianis, Demosthenes ; Stevens, David A. / Regulation of immune responses by T suppressor cells and by serum in chronic paracoccidioidomycosis. In: Cellular Immunology. 1988 ; Vol. 117, No. 1. pp. 1-11.
@article{d4a809707e9e45a29668b4b936ba8d74,
title = "Regulation of immune responses by T suppressor cells and by serum in chronic paracoccidioidomycosis",
abstract = "Regulation of cellular responses was studied during the course of chronic murine disseminated paracoccidioidomycosis. Regulation of peripheral blood lymphocyte (PBL) proliferative responses to concanavalin A (Con A) was studied in vitro by mixing PBL from infected and noninfected mice. PBL from mice infected for 18 weeks had depressed responses to Con A and they depressed the Con A responses of PBL from noninfected mice by 95{\%} when they were mixed in a 1:1 ratio. After treatment of PBL from infected mice with anti-Lyt-2.2 antibody plus complement, the responses to Con A were increased to normal values. The percentage of T-cell subpopulations in PBL from infected mice did not differ significantly from those of normal mice. Immunoregulation of delayed-type hypersensitivity (DTH) responses to antigen by serum from infected animals was studied in mice 1 week after intranasal (i.n.) infection, a time when DTH responses were maximal. DTH responses to antigen 7 days after i.n. infection (107 CFU Paracoccidioides brasiliensis) were significantly reduced when 0.5 ml of immune mouse serum (ELISA antibody titer to P. brasiliensis antigens 1:10,240) was given i.v. 1 day before infection (P < 0.01) or 1 day before skin testing (P < 0.001). Normal mouse serum did not have this effect. The results indicate that progression of chronic disseminated paracoccidioidomycosis was associated with the development of T-cell suppressor activity for Con A responses of PBL, and that DTH responses to antigen were depressed by the administration of serum with specific high titer antibodies.",
author = "Elizabeth Castaneda and Elmer Brummer and Demosthenes Pappagianis and Stevens, {David A.}",
year = "1988",
doi = "10.1016/0008-8749(88)90071-8",
language = "English (US)",
volume = "117",
pages = "1--11",
journal = "Cellular Immunology",
issn = "0008-8749",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Regulation of immune responses by T suppressor cells and by serum in chronic paracoccidioidomycosis

AU - Castaneda, Elizabeth

AU - Brummer, Elmer

AU - Pappagianis, Demosthenes

AU - Stevens, David A.

PY - 1988

Y1 - 1988

N2 - Regulation of cellular responses was studied during the course of chronic murine disseminated paracoccidioidomycosis. Regulation of peripheral blood lymphocyte (PBL) proliferative responses to concanavalin A (Con A) was studied in vitro by mixing PBL from infected and noninfected mice. PBL from mice infected for 18 weeks had depressed responses to Con A and they depressed the Con A responses of PBL from noninfected mice by 95% when they were mixed in a 1:1 ratio. After treatment of PBL from infected mice with anti-Lyt-2.2 antibody plus complement, the responses to Con A were increased to normal values. The percentage of T-cell subpopulations in PBL from infected mice did not differ significantly from those of normal mice. Immunoregulation of delayed-type hypersensitivity (DTH) responses to antigen by serum from infected animals was studied in mice 1 week after intranasal (i.n.) infection, a time when DTH responses were maximal. DTH responses to antigen 7 days after i.n. infection (107 CFU Paracoccidioides brasiliensis) were significantly reduced when 0.5 ml of immune mouse serum (ELISA antibody titer to P. brasiliensis antigens 1:10,240) was given i.v. 1 day before infection (P < 0.01) or 1 day before skin testing (P < 0.001). Normal mouse serum did not have this effect. The results indicate that progression of chronic disseminated paracoccidioidomycosis was associated with the development of T-cell suppressor activity for Con A responses of PBL, and that DTH responses to antigen were depressed by the administration of serum with specific high titer antibodies.

AB - Regulation of cellular responses was studied during the course of chronic murine disseminated paracoccidioidomycosis. Regulation of peripheral blood lymphocyte (PBL) proliferative responses to concanavalin A (Con A) was studied in vitro by mixing PBL from infected and noninfected mice. PBL from mice infected for 18 weeks had depressed responses to Con A and they depressed the Con A responses of PBL from noninfected mice by 95% when they were mixed in a 1:1 ratio. After treatment of PBL from infected mice with anti-Lyt-2.2 antibody plus complement, the responses to Con A were increased to normal values. The percentage of T-cell subpopulations in PBL from infected mice did not differ significantly from those of normal mice. Immunoregulation of delayed-type hypersensitivity (DTH) responses to antigen by serum from infected animals was studied in mice 1 week after intranasal (i.n.) infection, a time when DTH responses were maximal. DTH responses to antigen 7 days after i.n. infection (107 CFU Paracoccidioides brasiliensis) were significantly reduced when 0.5 ml of immune mouse serum (ELISA antibody titer to P. brasiliensis antigens 1:10,240) was given i.v. 1 day before infection (P < 0.01) or 1 day before skin testing (P < 0.001). Normal mouse serum did not have this effect. The results indicate that progression of chronic disseminated paracoccidioidomycosis was associated with the development of T-cell suppressor activity for Con A responses of PBL, and that DTH responses to antigen were depressed by the administration of serum with specific high titer antibodies.

UR - http://www.scopus.com/inward/record.url?scp=0024273536&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024273536&partnerID=8YFLogxK

U2 - 10.1016/0008-8749(88)90071-8

DO - 10.1016/0008-8749(88)90071-8

M3 - Article

C2 - 2972392

AN - SCOPUS:0024273536

VL - 117

SP - 1

EP - 11

JO - Cellular Immunology

JF - Cellular Immunology

SN - 0008-8749

IS - 1

ER -