Regulation of granulosa cell proliferation and EGF-like ligands during the periovulatory interval in monkeys

K. N. Fru, M. Cherian-Shaw, M. Puttabyatappa, C. A. VandeVoort, C. L. Chaffin

Research output: Contribution to journalArticlepeer-review

34 Scopus citations


Background: This study seeks to clarify cell cycle dynamics of granulosa cells following hCG and elucidate the expression of epidermal growth factor (EGF)-like ligands during luteinization. Methods: Granulosa cells were obtained from rhesus macaques undergoing controlled ovarian stimulation protocols before or after an ovulatory hCG bolus. Cell cycle characteristics were determined by flow cytometry and levels of EGF receptor (EGFR), amphiregulin (AREG), epiregulin (EREG) and betacellulin (BTC) mRNAs were measured by real-time RT-PCR. Results: The proportion of cells in S-phase was 7.5% prior to hCG and did not decline until 24 h after hCG (3.1%). EGFR protein and BTC mRNA did not change following hCG, whereas AREG and EREG mRNA increased starting at 3 and 12 h post-hCG, respectively, and remained elevated thereafter. Conclusions: Cell cycle transit of macaque granulosa cells does not change until 24 h after an ovulatory stimulus, whereas the EGF-like ligands EREG and AREG are increased rapidly. This suggests that luteinizing granulosa cells are refractory to mitogenic stimulation by EGFR ligands.

Original languageEnglish (US)
Pages (from-to)1247-1252
Number of pages6
JournalHuman Reproduction
Issue number5
StatePublished - May 2007


  • Epidermal growth factor
  • Granulosa cell
  • Luteinization
  • Non-human primate
  • Proliferation

ASJC Scopus subject areas

  • Physiology
  • Developmental Biology
  • Obstetrics and Gynecology
  • Reproductive Medicine


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