Regulation of gene expression in RAW 264.7 macrophage cell line by interferon-γ

Rama S. Kota, John C Rutledge, Kishorchandra Gohil, Aseem Kumar, Richard I. Enelow, Chilakamarti V. Ramana

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

Macrophages play an important role in immune responses and in inflammatory disease states such as atherosclerosis. Interferon-γ (IFN-γ) is a major cytokine involved in the activation of macrophages. To elucidate the primary response of various genes and biological pathways regulated by IFN-γ in macrophage, we analyzed the gene expression profile in RAW 264.7 macrophage cells treated with IFN-γ for 4 h. Microarray analysis revealed that about 400 genes were differentially expressed, of which about 250 genes were up-regulated and 150 were down-regulated. Functional organization of the transcriptome revealed that induced genes are involved in antimicrobial and antiviral responses, antigen presentation, chemokine and cytokine signaling, and inhibition of cell growth. We also found that expression of genes involved in cell-cycle control, DNA repair, and lipid metabolism was suppressed by IFN-γ. We also identified induction of multiple transcription factors by IFN-γ in RAW 264.7 cells. Functional annotation of genes regulated by IFN-γ in RAW 264.7 cells may provide novel insights into the role of macrophages in immunity and in inflammatory disease.

Original languageEnglish (US)
Pages (from-to)1137-1146
Number of pages10
JournalBiochemical and Biophysical Research Communications
Volume342
Issue number4
DOIs
StatePublished - Apr 21 2006

Keywords

  • IFN-γ
  • Inflammation
  • Macrophage
  • Microarrays
  • RAW 264.7
  • Stat1

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Fingerprint Dive into the research topics of 'Regulation of gene expression in RAW 264.7 macrophage cell line by interferon-γ'. Together they form a unique fingerprint.

  • Cite this