Abstract
Macrophages play an important role in immune responses and in inflammatory disease states such as atherosclerosis. Interferon-γ (IFN-γ) is a major cytokine involved in the activation of macrophages. To elucidate the primary response of various genes and biological pathways regulated by IFN-γ in macrophage, we analyzed the gene expression profile in RAW 264.7 macrophage cells treated with IFN-γ for 4 h. Microarray analysis revealed that about 400 genes were differentially expressed, of which about 250 genes were up-regulated and 150 were down-regulated. Functional organization of the transcriptome revealed that induced genes are involved in antimicrobial and antiviral responses, antigen presentation, chemokine and cytokine signaling, and inhibition of cell growth. We also found that expression of genes involved in cell-cycle control, DNA repair, and lipid metabolism was suppressed by IFN-γ. We also identified induction of multiple transcription factors by IFN-γ in RAW 264.7 cells. Functional annotation of genes regulated by IFN-γ in RAW 264.7 cells may provide novel insights into the role of macrophages in immunity and in inflammatory disease.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1137-1146 |
| Number of pages | 10 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 342 |
| Issue number | 4 |
| DOIs | |
| State | Published - Apr 21 2006 |
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Keywords
- IFN-γ
- Inflammation
- Macrophage
- Microarrays
- RAW 264.7
- Stat1
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Regulation of gene expression in RAW 264.7 macrophage cell line by interferon-γ. / Kota, Rama S.; Rutledge, John C; Gohil, Kishorchandra; Kumar, Aseem; Enelow, Richard I.; Ramana, Chilakamarti V.
In: Biochemical and Biophysical Research Communications, Vol. 342, No. 4, 21.04.2006, p. 1137-1146.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Regulation of gene expression in RAW 264.7 macrophage cell line by interferon-γ
AU - Kota, Rama S.
AU - Rutledge, John C
AU - Gohil, Kishorchandra
AU - Kumar, Aseem
AU - Enelow, Richard I.
AU - Ramana, Chilakamarti V.
PY - 2006/4/21
Y1 - 2006/4/21
N2 - Macrophages play an important role in immune responses and in inflammatory disease states such as atherosclerosis. Interferon-γ (IFN-γ) is a major cytokine involved in the activation of macrophages. To elucidate the primary response of various genes and biological pathways regulated by IFN-γ in macrophage, we analyzed the gene expression profile in RAW 264.7 macrophage cells treated with IFN-γ for 4 h. Microarray analysis revealed that about 400 genes were differentially expressed, of which about 250 genes were up-regulated and 150 were down-regulated. Functional organization of the transcriptome revealed that induced genes are involved in antimicrobial and antiviral responses, antigen presentation, chemokine and cytokine signaling, and inhibition of cell growth. We also found that expression of genes involved in cell-cycle control, DNA repair, and lipid metabolism was suppressed by IFN-γ. We also identified induction of multiple transcription factors by IFN-γ in RAW 264.7 cells. Functional annotation of genes regulated by IFN-γ in RAW 264.7 cells may provide novel insights into the role of macrophages in immunity and in inflammatory disease.
AB - Macrophages play an important role in immune responses and in inflammatory disease states such as atherosclerosis. Interferon-γ (IFN-γ) is a major cytokine involved in the activation of macrophages. To elucidate the primary response of various genes and biological pathways regulated by IFN-γ in macrophage, we analyzed the gene expression profile in RAW 264.7 macrophage cells treated with IFN-γ for 4 h. Microarray analysis revealed that about 400 genes were differentially expressed, of which about 250 genes were up-regulated and 150 were down-regulated. Functional organization of the transcriptome revealed that induced genes are involved in antimicrobial and antiviral responses, antigen presentation, chemokine and cytokine signaling, and inhibition of cell growth. We also found that expression of genes involved in cell-cycle control, DNA repair, and lipid metabolism was suppressed by IFN-γ. We also identified induction of multiple transcription factors by IFN-γ in RAW 264.7 cells. Functional annotation of genes regulated by IFN-γ in RAW 264.7 cells may provide novel insights into the role of macrophages in immunity and in inflammatory disease.
KW - IFN-γ
KW - Inflammation
KW - Macrophage
KW - Microarrays
KW - RAW 264.7
KW - Stat1
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U2 - 10.1016/j.bbrc.2006.02.087
DO - 10.1016/j.bbrc.2006.02.087
M3 - Article
C2 - 16516165
AN - SCOPUS:33644912011
VL - 342
SP - 1137
EP - 1146
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 4
ER -