Regulation of eukaryotic translation initiation factor expression during T-cell activation

Thomas R. Boal, John A. Chiorini, Roger B. Cohen, Suzanne Miyamoto, Robert M. Frederickson, Nahum Sonenberg, Brian Safer

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Primary T-cells are metabolically quiescent, with little DNA, RNA or protein synthesis. Upon mitogenic stimulation the rate of protein synthesis increases 10-fold. We have studied the role of eIF-2 and eIF-4α (eIF-4E) expression in the mechanism of translational activation. During this period, the levels of eIF-2α and eIF-4α mRNA increase some 50-fold. Similar to the increase in ribosomes and mRNA, the number of eIF-2α, eIF-2β, and eIF-4α molecules per cell also increase 2-3-fold. This suggests that in addition to an increase in the pool size of translational components, an additional mechanism exists which results in an increased efficiency of factor utilization. We have looked at initiation factor phosphorylation. We find that eIF-2α does not undergo significant changes in its phosphorylation state nor is there a change in the efficiency of eIF-2 utilization. However, there is a rapid increase in the phosphorylation state of eIF-4α which correlates with the rapid increase in translational activity. It thus appears there are 2 distinct components responsible for the translational activation of quiescent T-cells during mitogenic stimulation. The first is the phosphorylation of eIF-4α, with a concomitant increase in the efficiency of eIF-4α utilization. The second is an increase in the pool sizes of eIF-2 and eIF-4α.

Original languageEnglish (US)
Pages (from-to)257-264
Number of pages8
JournalBBA - Molecular Cell Research
Volume1176
Issue number3
DOIs
StatePublished - Apr 16 1993
Externally publishedYes

Fingerprint

Eukaryotic Initiation Factors
T-Lymphocytes
Phosphorylation
Peptide Initiation Factors
Messenger RNA
eIF-4
Ribosomes
Proteins
RNA
DNA

Keywords

  • eIF-2α
  • eIF-4α
  • Mitogen
  • Phosphorylation
  • T cell
  • Translation

ASJC Scopus subject areas

  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Boal, T. R., Chiorini, J. A., Cohen, R. B., Miyamoto, S., Frederickson, R. M., Sonenberg, N., & Safer, B. (1993). Regulation of eukaryotic translation initiation factor expression during T-cell activation. BBA - Molecular Cell Research, 1176(3), 257-264. https://doi.org/10.1016/0167-4889(93)90053-R

Regulation of eukaryotic translation initiation factor expression during T-cell activation. / Boal, Thomas R.; Chiorini, John A.; Cohen, Roger B.; Miyamoto, Suzanne; Frederickson, Robert M.; Sonenberg, Nahum; Safer, Brian.

In: BBA - Molecular Cell Research, Vol. 1176, No. 3, 16.04.1993, p. 257-264.

Research output: Contribution to journalArticle

Boal, TR, Chiorini, JA, Cohen, RB, Miyamoto, S, Frederickson, RM, Sonenberg, N & Safer, B 1993, 'Regulation of eukaryotic translation initiation factor expression during T-cell activation', BBA - Molecular Cell Research, vol. 1176, no. 3, pp. 257-264. https://doi.org/10.1016/0167-4889(93)90053-R
Boal TR, Chiorini JA, Cohen RB, Miyamoto S, Frederickson RM, Sonenberg N et al. Regulation of eukaryotic translation initiation factor expression during T-cell activation. BBA - Molecular Cell Research. 1993 Apr 16;1176(3):257-264. https://doi.org/10.1016/0167-4889(93)90053-R
Boal, Thomas R. ; Chiorini, John A. ; Cohen, Roger B. ; Miyamoto, Suzanne ; Frederickson, Robert M. ; Sonenberg, Nahum ; Safer, Brian. / Regulation of eukaryotic translation initiation factor expression during T-cell activation. In: BBA - Molecular Cell Research. 1993 ; Vol. 1176, No. 3. pp. 257-264.
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