Regulation of ceramide synthase by casein kinase 2-dependent phosphorylation in Saccharomyces cerevisiae

Tara Fresques, Brad Niles, Sofia Aronova, Huzefa Mogri, Taha Rakhshandehroo, Ted Powers

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Complex sphingolipids are important components of eukaryotic cell membranes and, together with their biosynthetic precursors, including sphingoid long chain bases and ceramides, have important signaling functions crucial for cell growth and survival. Ceramides are produced at the endoplasmic reticulum (ER) membrane by a multicomponent enzyme complex termed ceramide synthase (CerS). In budding yeast, this complex is composed of two catalytic subunits, Lac1 and Lag1, as well as an essential regulatory subunit, Lip1. Proper formation of ceramides by CerS has been shown previously to require the Cka2 subunit of casein kinase 2 (CK2), a ubiquitous enzyme with multiple cellular functions, but the precise mechanism involved has remained unidentified. Here we present evidence that Lac1 and Lag1 are direct targets for CK2 and that phosphorylation at conserved positions within the C-terminal cytoplasmic domain of each protein is required for optimal CerS activity. Our data suggest that phosphorylation of Lac1 and Lag1 is important for proper localization and distribution of CerS within the ER membrane and that phosphorylation of these sites is functionally linked to the COP I-dependent C-terminal dilysine ER retrieval pathway. Together, our data identify CK2 as an important regulator of sphingolipid metabolism, and additionally, because both ceramides and CK2 have been implicated in the regulation of cancer, our findings may lead to an enhanced understanding of their relationship in health and disease.

Original languageEnglish (US)
Pages (from-to)1395-1403
Number of pages9
JournalJournal of Biological Chemistry
Volume290
Issue number3
DOIs
StatePublished - Jan 16 2015

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Casein Kinase II
Phosphorylation
Yeast
Saccharomyces cerevisiae
Ceramides
Endoplasmic Reticulum
Sphingolipids
lysyllysine
Coat Protein Complex I
Membranes
Saccharomycetales
Cell growth
Eukaryotic Cells
Enzymes
Cell membranes
Metabolism
Catalytic Domain
Cell Survival
Cell Membrane
Health

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Regulation of ceramide synthase by casein kinase 2-dependent phosphorylation in Saccharomyces cerevisiae. / Fresques, Tara; Niles, Brad; Aronova, Sofia; Mogri, Huzefa; Rakhshandehroo, Taha; Powers, Ted.

In: Journal of Biological Chemistry, Vol. 290, No. 3, 16.01.2015, p. 1395-1403.

Research output: Contribution to journalArticle

Fresques, T, Niles, B, Aronova, S, Mogri, H, Rakhshandehroo, T & Powers, T 2015, 'Regulation of ceramide synthase by casein kinase 2-dependent phosphorylation in Saccharomyces cerevisiae', Journal of Biological Chemistry, vol. 290, no. 3, pp. 1395-1403. https://doi.org/10.1074/jbc.M114.621086
Fresques, Tara ; Niles, Brad ; Aronova, Sofia ; Mogri, Huzefa ; Rakhshandehroo, Taha ; Powers, Ted. / Regulation of ceramide synthase by casein kinase 2-dependent phosphorylation in Saccharomyces cerevisiae. In: Journal of Biological Chemistry. 2015 ; Vol. 290, No. 3. pp. 1395-1403.
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