Regulation of androgen and vitamin D receptors by 1,25-dihydroxyvitamin D3 in human prostate epithelial and stromal cells

Eddy S. Leman, Fernando DeMiguel, Allen C Gao, Robert H. Getzenberg

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Purpose: The mechanisms of the interaction between 1,25-dihydroxyvitamin D3 (1,25 D) and androgens, and their respective receptors in their action on the prostate are not completely understood. We examined the interplay of 1,25 D and androgens on the epithelial and stromal cells of the prostate. Materials and Methods: The human neonatal prostatic epithelial cell line 267B-1 (BRFF, Inc., Ijamsville, Maryland) and primary cultures of human prostate stromal cells were treated with medium containing 5 or 10 μM 1,25 D or ethanol (control) in the presence or absence of 10 nM dihydrotestosterone (DHT) (Sigma Chemical Co., St. Louis, Missouri). Protein levels of androgen receptor (AR) and vitamin D receptor (VDR) were determined by immunoblot analysis of whole cell extracts. Electrophoresis mobility shift assays were used to determine AR and VDR DNA binding activities. Results: The VDR protein level of 267B-1 cells was increased in the presence of 1,25 D (with the maximum effects seen at 24 hours) regardless of the presence or absence of DHT. In addition, exogenous DHT increased the AR and VDR DNA binding activities of 267B-1 and stromal cells in the presence of 1,25 D. Conclusions: ARs in the normal prostate are regulated by androgens, whereas VDRs in the normal prostate can be regulated by 1,25 D as well as by other androgens such as testosterone. This finding further supports the concept that 1,25 D as a steroid hormone, in addition to other androgens such as DHT, may have a role in the growth and differentiation of normal prostate.

Original languageEnglish (US)
Pages (from-to)235-240
Number of pages6
JournalJournal of Urology
Volume170
Issue number1
DOIs
StatePublished - Jul 1 2003
Externally publishedYes

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Keywords

  • Androgens
  • Calcitriol
  • Prostate
  • Receptors, androgens
  • Steroids

ASJC Scopus subject areas

  • Urology

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