Regulation and intracellular localization of Saccharomyces cerevisiae strand exchange protein 1 (Sep1/Xrn1/Kem1), a multifunctional exonuclease

Wolf Dietrich Heyer, Arlen W. Johnson, Ursula Reinhart, Richard D. Kolodner

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

The Saccharomyces cerevisiae strand exchange protein 1 (Sep1; also referred to as Xrn1, Kem1, Rar5, or Stpβ) catalyzes the formation of hybrid DNA from model substrates in vitro. The protein is also a 5'-to-3' exonuclease active on DNA and RNA. Multiple roles for the in vivo function of Sep1, ranging from DNA recombination and cytoskeleton to RNA turnover, have been proposed. We show that Sep1 is an abundant protein in vegetative S. cerevisiae cells, present at about 80,000 molecules per diploid cell. Protein levels were not changed during the cell cycle or in response to DNA-damaging agents but increased twofold during meiosis. Cell fractionation and indirect immunofluorescence studies indicated that > 90% of Sep1 was cytoplasmic in vegetative cells, and indirect immunofluorescence indicated a cytoplasmic localization in meiotic cells as well. The localization supports the proposal that Sep1 has a role in cytoplasmic RNA metabolism. Anti-Sep1 monoclonal antibodies detected cross-reacting antigens in the fission yeast Schizosaccharomyces pombe, in Drosophila melanogaster embryos, in Xenopus laevis, and in a mouse pre-B-cell line.

Original languageEnglish (US)
Pages (from-to)2728-2736
Number of pages9
JournalMolecular and Cellular Biology
Volume15
Issue number5
StatePublished - May 1995
Externally publishedYes

Fingerprint

Exonucleases
Saccharomyces cerevisiae
Schizosaccharomyces
DNA
RNA
Indirect Fluorescent Antibody Technique
Proteins
Cell Fractionation
B-Lymphoid Precursor Cells
Xenopus laevis
Meiosis
Drosophila melanogaster
Cytoskeleton
Diploidy
Genetic Recombination
Cell Cycle
Embryonic Structures
Monoclonal Antibodies
Antigens
Cell Line

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Regulation and intracellular localization of Saccharomyces cerevisiae strand exchange protein 1 (Sep1/Xrn1/Kem1), a multifunctional exonuclease. / Heyer, Wolf Dietrich; Johnson, Arlen W.; Reinhart, Ursula; Kolodner, Richard D.

In: Molecular and Cellular Biology, Vol. 15, No. 5, 05.1995, p. 2728-2736.

Research output: Contribution to journalArticle

Heyer, Wolf Dietrich ; Johnson, Arlen W. ; Reinhart, Ursula ; Kolodner, Richard D. / Regulation and intracellular localization of Saccharomyces cerevisiae strand exchange protein 1 (Sep1/Xrn1/Kem1), a multifunctional exonuclease. In: Molecular and Cellular Biology. 1995 ; Vol. 15, No. 5. pp. 2728-2736.
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