Regrowth of Serotonin Axons in the Adult Mouse Brain Following Injury

Yunju Jin, Sarah E. Dougherty, Kevin Wood, Landy Sun, Robert H. Cudmore, Aya Abdalla, Geetha Kannan, Mikhail Pletnikov, Parastoo Hashemi, David J. Linden

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

It is widely believed that damaged axons in the adult mammalian brain have little capacity to regrow, thereby impeding functional recovery after injury. Studies using fixed tissue have suggested that serotonin neurons might be a notable exception, but remain inconclusive. We have employed in vivo two-photon microscopy to produce time-lapse images of serotonin axons in the neocortex of the adult mouse. Serotonin axons undergo massive retrograde degeneration following amphetamine treatment and subsequent slow recovery of axonal density, which is dominated by new growth with little contribution from local sprouting. A stab injury that transects serotonin axons running in the neocortex is followed by local regression of cut serotonin axons and followed by regrowth from cut ends into and across the stab rift zone. Regrowing serotonin axons do not follow the pathways left by degenerated axons. The regrown axons release serotonin and their regrowth is correlated with recovery in behavioral tests.

Original languageEnglish (US)
Pages (from-to)748-762
Number of pages15
JournalNeuron
Volume91
Issue number4
DOIs
StatePublished - Aug 17 2016
Externally publishedYes

Fingerprint

Brain Injuries
Axons
Serotonin
Neocortex
Retrograde Degeneration
Wounds and Injuries
Amphetamine
Photons
Running
Microscopy
Neurons
Brain
Growth

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Jin, Y., Dougherty, S. E., Wood, K., Sun, L., Cudmore, R. H., Abdalla, A., ... Linden, D. J. (2016). Regrowth of Serotonin Axons in the Adult Mouse Brain Following Injury. Neuron, 91(4), 748-762. https://doi.org/10.1016/j.neuron.2016.07.024

Regrowth of Serotonin Axons in the Adult Mouse Brain Following Injury. / Jin, Yunju; Dougherty, Sarah E.; Wood, Kevin; Sun, Landy; Cudmore, Robert H.; Abdalla, Aya; Kannan, Geetha; Pletnikov, Mikhail; Hashemi, Parastoo; Linden, David J.

In: Neuron, Vol. 91, No. 4, 17.08.2016, p. 748-762.

Research output: Contribution to journalArticle

Jin, Y, Dougherty, SE, Wood, K, Sun, L, Cudmore, RH, Abdalla, A, Kannan, G, Pletnikov, M, Hashemi, P & Linden, DJ 2016, 'Regrowth of Serotonin Axons in the Adult Mouse Brain Following Injury', Neuron, vol. 91, no. 4, pp. 748-762. https://doi.org/10.1016/j.neuron.2016.07.024
Jin, Yunju ; Dougherty, Sarah E. ; Wood, Kevin ; Sun, Landy ; Cudmore, Robert H. ; Abdalla, Aya ; Kannan, Geetha ; Pletnikov, Mikhail ; Hashemi, Parastoo ; Linden, David J. / Regrowth of Serotonin Axons in the Adult Mouse Brain Following Injury. In: Neuron. 2016 ; Vol. 91, No. 4. pp. 748-762.
@article{378513d15c724734893af797c0485f01,
title = "Regrowth of Serotonin Axons in the Adult Mouse Brain Following Injury",
abstract = "It is widely believed that damaged axons in the adult mammalian brain have little capacity to regrow, thereby impeding functional recovery after injury. Studies using fixed tissue have suggested that serotonin neurons might be a notable exception, but remain inconclusive. We have employed in vivo two-photon microscopy to produce time-lapse images of serotonin axons in the neocortex of the adult mouse. Serotonin axons undergo massive retrograde degeneration following amphetamine treatment and subsequent slow recovery of axonal density, which is dominated by new growth with little contribution from local sprouting. A stab injury that transects serotonin axons running in the neocortex is followed by local regression of cut serotonin axons and followed by regrowth from cut ends into and across the stab rift zone. Regrowing serotonin axons do not follow the pathways left by degenerated axons. The regrown axons release serotonin and their regrowth is correlated with recovery in behavioral tests.",
author = "Yunju Jin and Dougherty, {Sarah E.} and Kevin Wood and Landy Sun and Cudmore, {Robert H.} and Aya Abdalla and Geetha Kannan and Mikhail Pletnikov and Parastoo Hashemi and Linden, {David J.}",
year = "2016",
month = "8",
day = "17",
doi = "10.1016/j.neuron.2016.07.024",
language = "English (US)",
volume = "91",
pages = "748--762",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "4",

}

TY - JOUR

T1 - Regrowth of Serotonin Axons in the Adult Mouse Brain Following Injury

AU - Jin, Yunju

AU - Dougherty, Sarah E.

AU - Wood, Kevin

AU - Sun, Landy

AU - Cudmore, Robert H.

AU - Abdalla, Aya

AU - Kannan, Geetha

AU - Pletnikov, Mikhail

AU - Hashemi, Parastoo

AU - Linden, David J.

PY - 2016/8/17

Y1 - 2016/8/17

N2 - It is widely believed that damaged axons in the adult mammalian brain have little capacity to regrow, thereby impeding functional recovery after injury. Studies using fixed tissue have suggested that serotonin neurons might be a notable exception, but remain inconclusive. We have employed in vivo two-photon microscopy to produce time-lapse images of serotonin axons in the neocortex of the adult mouse. Serotonin axons undergo massive retrograde degeneration following amphetamine treatment and subsequent slow recovery of axonal density, which is dominated by new growth with little contribution from local sprouting. A stab injury that transects serotonin axons running in the neocortex is followed by local regression of cut serotonin axons and followed by regrowth from cut ends into and across the stab rift zone. Regrowing serotonin axons do not follow the pathways left by degenerated axons. The regrown axons release serotonin and their regrowth is correlated with recovery in behavioral tests.

AB - It is widely believed that damaged axons in the adult mammalian brain have little capacity to regrow, thereby impeding functional recovery after injury. Studies using fixed tissue have suggested that serotonin neurons might be a notable exception, but remain inconclusive. We have employed in vivo two-photon microscopy to produce time-lapse images of serotonin axons in the neocortex of the adult mouse. Serotonin axons undergo massive retrograde degeneration following amphetamine treatment and subsequent slow recovery of axonal density, which is dominated by new growth with little contribution from local sprouting. A stab injury that transects serotonin axons running in the neocortex is followed by local regression of cut serotonin axons and followed by regrowth from cut ends into and across the stab rift zone. Regrowing serotonin axons do not follow the pathways left by degenerated axons. The regrown axons release serotonin and their regrowth is correlated with recovery in behavioral tests.

UR - http://www.scopus.com/inward/record.url?scp=84991066538&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84991066538&partnerID=8YFLogxK

U2 - 10.1016/j.neuron.2016.07.024

DO - 10.1016/j.neuron.2016.07.024

M3 - Article

C2 - 27499084

AN - SCOPUS:84991066538

VL - 91

SP - 748

EP - 762

JO - Neuron

JF - Neuron

SN - 0896-6273

IS - 4

ER -