The serial development of cell mediated immunity (CMI), cytotoxic antibody activity, serum blocking activity, and virus neutralizing antibody levels were monitored in vitro for beagle and mongrel puppies inoculated with feline sarcoma virus (FeSV) and were compared to in vivo histologic markers of regression of induced sarcomas. CMI developed rapidly and maintained a high level of in vitro activity throughout the tumor life span. Cytotoxic antibody levels similarly rose rapidly to peak just before clinically detectable regression and then declined during most of the regression sequence. This suggested antibody fixation at the tumor site, correlating with the histologic findings of focal necrosis and neutrophilic infiltrates. Inactivation of antibody by circulating antigen with subsequent immune complex formation was a possibility. Levels of virus neutralizing antibody in sera paralleled those of cytotoxic antibody; their relationship in the circulation was not clear, but each related to virus determined antigenic specificities. Serum blocking activity rose rapidly, leveled off during most of the tumor life span, and rose slightly during the last stages of regression. This partly explained the lack of in vivo tumor lymphoid infiltrates to correlate with the striking in vitro CMI. Blocking activity was also present, however, when lymphoid infiltrates were seen histologically. Thus, in vitro in vivo correlation was best for cytotoxic antibody, which suggested that antigen antibody reactions involving neutrophil mediated regression sequences were important in effecting tumor cell destruction.
|Original language||English (US)|
|Number of pages||7|
|Journal||Journal of the National Cancer Institute|
|State||Published - Dec 1 1975|
ASJC Scopus subject areas
- Cancer Research