Regioselective One-Pot Multienzyme (OPME) Chemoenzymatic Strategies for Systematic Synthesis of Sialyl Core 2 Glycans

Abhishek Santra, Yanhong Li, Tamashree Ghosh, Riyao Li, Hai Yu, Xi Chen

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

O-GalNAc glycans or mucin-type glycans are common protein post-translational modifications in eukaryotes. Core 2 O-GalNAc glycans are branched structures that are broadly distributed in glycoproteins and mucins of all types of cells. To better understand their biological roles, it is important to obtain structurally defined Core 2 O-GalNAc glycans. We present here regioselective one-pot multienzyme (OPME) chemoenzymatic strategies to systematically access a diverse array of sialyl Core 2 glycans. Regioselectivity can be achieved by using OPME systems containing a glycosyltransferase with restricted acceptor specificity or by differentiating the branches using altered glycosylation sequences. This work provides a general regioselective strategy to access diverse Core 2 O-GalNAc glycans, which can be extended for the synthesis of other complex branched glycans.

Original languageEnglish (US)
Pages (from-to)211-215
Number of pages5
JournalACS Catalysis
DOIs
StateAccepted/In press - Jan 1 2018

Keywords

  • biocatalysis
  • enzymatic synthesis
  • glycosyltransferase
  • O-GalNAc glycan
  • sialyltransferase

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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