Regional assignment and tissue expression of twenty-three expressed sequence tags (ESTs) from human chromosome 5

Tamara V. Feldblyum, Donna R. Maglott, John Douglas Mcpherson, Mark Adams, Barbara L. Apostol, A. Scott Durkin, John J. Wasmuth, William C. Nierman

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Regional localization and expression patterns are reported for 19 expressed sequence tags (ESTs) from human chromosome 5, two of which were derived from the same transcript. Two of the ESTs correspond to genes not previously characterized in humans: a stress-activated protein kinase and nicotinamide nucleotide transhydrogenase. Expression was determined by three methods: Northern blots, PCR from tissue-specific cDNA libraries, and sequence sampling from EST sequencing projects. Six of the ESTs show no expression, and EST01986 appears to be expressed predominantly in the brain by all methods tested.

Original languageEnglish (US)
Pages (from-to)128-130
Number of pages3
JournalGenomics
Volume33
Issue number1
DOIs
StatePublished - Apr 1 1996

ASJC Scopus subject areas

  • Genetics

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    Feldblyum, T. V., Maglott, D. R., Mcpherson, J. D., Adams, M., Apostol, B. L., Durkin, A. S., Wasmuth, J. J., & Nierman, W. C. (1996). Regional assignment and tissue expression of twenty-three expressed sequence tags (ESTs) from human chromosome 5. Genomics, 33(1), 128-130. https://doi.org/10.1006/geno.1996.0169