Abstract
Whole-cell patch-clamp recordings were made from isolated goldfish retinal ganglion cell somata. Sodium- and calcium-dependent action potentials-blocked with tetrodotoxin (TTX) and Co+ ions, respectively-were evoked under current-clamp. Depolarization of these somata under voltage clamp activated distinguishable Na+ and Ca2+ conductances: the former was permeable to Na+, blocked by TTX, impermeable to N-methyl-d-glucamine and tris(hydroxymethyl)aminomethane, and began to activate around -45 mV; the latter were permeable to Ca2+, but not Co2+, and began to activate around -55 mV. These results are consistent with recordings from carp, frog, salamander and rat retinal ganglion cells.
Original language | English (US) |
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Pages (from-to) | 477-485 |
Number of pages | 9 |
Journal | Vision Research |
Volume | 31 |
Issue number | 3 |
DOIs | |
State | Published - 1991 |
Keywords
- Action potentials
- Calcium currents
- Retinal ganglion cells
- Sodium currents
- Whole-cell patch clamp
ASJC Scopus subject areas
- Ophthalmology
- Sensory Systems