Regeneration of enteric ganglia in mechanically lengthened jejunum after restoration into intestinal continuity

Ziyad Jabaji, Rebecca Stark, James C.Y. Dunn

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Purpose: We previously demonstrated that it is feasible to lengthen intestinal segments with mechanical force and to restore them back into intestinal continuity. The changes in the enteric ganglia in the lengthened intestinal segments have not been described. Methods: A 1-cm segment of rodent jejunum was isolated from intestinal continuity and was lengthened using a spring. After lengthening, jejunal segments were either retrieved (n = 4) or restored into intestinal continuity (n = 4). Rats with restored segments were euthanized 2 to 3 weeks later. Ganglia were identified and quantified by immunostaining of histological sections. Results: The normal jejunum had 51 ± 5 myenteric and 31 ± 2 submucosal ganglia per circumferential tissue section. Lengthened segments had 21 ± 7 myenteric and 2 ± 2 submucosal ganglia. Restored segments had 46 ± 14 myenteric and 10 ± 10 submucosal ganglia. Circumferential density of ganglia followed a similar pattern. Conclusion: Mechanical lengthening led to a paucity of submucosal and myenteric ganglia. After restoration into continuity, the number increased toward normal, indicating regeneration of the enteric ganglia. The function of regenerated ganglia needs to be assessed in the future.

Original languageEnglish (US)
Pages (from-to)118-123
Number of pages6
JournalJournal of Pediatric Surgery
Volume48
Issue number1
DOIs
StatePublished - Jan 1 2013

Keywords

  • Distraction enterogenesis
  • Enteric nervous system
  • Ganglia
  • Mechanical enterogenesis
  • Short bowel syndrome

ASJC Scopus subject areas

  • Surgery
  • Pediatrics, Perinatology, and Child Health

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