Reflux aspiration in lungs of dogs with respiratory disease and in healthy West Highland White Terriers

O. L.M. Määttä, H. P. Laurila, S. Holopainen, L. Lilja-Maula, M. Melamies, S. J. Viitanen, Lynelle R Johnson, N. Koho, M. Neuvonen, M. Niemi, M. M. Rajamäki

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: Gastroesophageal reflux and microaspiration (MA) of gastric juice are associated with various human respiratory diseases but not in dogs. Objective: To detect the presence of bile acids in bronchoalveolar lavage fluid (BALF) of dogs with various respiratory diseases. Animals: Twenty-seven West Highland White Terriers (WHWTs) with canine idiopathic pulmonary fibrosis (CIPF), 11 dogs with bacterial pneumonia (BP), 13 with chronic bronchitis (CB), 9 with eosinophilic bronchopneumopathy (EBP), 19 with laryngeal dysfunction (LD), 8 Irish Wolfhounds (IWHs) with previous BPs, 13 healthy WHWTs, all privately owned dogs, and 6 healthy research colony Beagles. Methods: Prospective cross-sectional observational study with convenience sampling of dogs. Bile acids were measured by mass spectrometry in BALF samples. Total bile acid (TBA) concentration was calculated as a sum of 17 different bile acids. Results: Concentrations of TBA were above the limit of quantification in 78% of CIPF, 45% of BP, 62% of CB, 44% of EBP, 68% of LD, and 13% of IWH dogs. In healthy dogs, bile acids were detected less commonly in Beagles (0/6) than in healthy WHWTs (10/13). Concentrations of TBA were significantly higher in CIPF (median 0.013 μM, range not quantifiable [n.q.]-0.14 μM, P <.001), healthy WHWTs (0.0052 μM, n.q.-1.2 μM, P =.003), LD (0.010 μM, n.q.-2.3 μM, P =.015), and CB (0.0078 μM, n.q.-0.073 μM, P =.018) groups compared to Beagles (0 μM, n.q.). Conclusion and Clinical Importance: These results suggest that MA occurs in various respiratory diseases of dogs and also in healthy WHWTs.

Original languageEnglish (US)
Article numbere15321
JournalJournal of Veterinary Internal Medicine
DOIs
StateAccepted/In press - Jan 1 2018

Fingerprint

terriers
Bile Acids and Salts
respiratory tract diseases
highlands
lungs
Dogs
bile acids
Lung
dogs
Idiopathic Pulmonary Fibrosis
Chronic Bronchitis
Canidae
Bacterial Pneumonia
bronchitis
Bronchoalveolar Lavage Fluid
Beagle
fibrosis
bacterial pneumonia
Dog Diseases
Gastric Juice

Keywords

  • bile acid
  • bronchoalveolar lavage fluid
  • canine
  • microaspiration

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Määttä, O. L. M., Laurila, H. P., Holopainen, S., Lilja-Maula, L., Melamies, M., Viitanen, S. J., ... Rajamäki, M. M. (Accepted/In press). Reflux aspiration in lungs of dogs with respiratory disease and in healthy West Highland White Terriers. Journal of Veterinary Internal Medicine, [e15321]. https://doi.org/10.1111/jvim.15321

Reflux aspiration in lungs of dogs with respiratory disease and in healthy West Highland White Terriers. / Määttä, O. L.M.; Laurila, H. P.; Holopainen, S.; Lilja-Maula, L.; Melamies, M.; Viitanen, S. J.; Johnson, Lynelle R; Koho, N.; Neuvonen, M.; Niemi, M.; Rajamäki, M. M.

In: Journal of Veterinary Internal Medicine, 01.01.2018.

Research output: Contribution to journalArticle

Määttä, OLM, Laurila, HP, Holopainen, S, Lilja-Maula, L, Melamies, M, Viitanen, SJ, Johnson, LR, Koho, N, Neuvonen, M, Niemi, M & Rajamäki, MM 2018, 'Reflux aspiration in lungs of dogs with respiratory disease and in healthy West Highland White Terriers', Journal of Veterinary Internal Medicine. https://doi.org/10.1111/jvim.15321
Määttä, O. L.M. ; Laurila, H. P. ; Holopainen, S. ; Lilja-Maula, L. ; Melamies, M. ; Viitanen, S. J. ; Johnson, Lynelle R ; Koho, N. ; Neuvonen, M. ; Niemi, M. ; Rajamäki, M. M. / Reflux aspiration in lungs of dogs with respiratory disease and in healthy West Highland White Terriers. In: Journal of Veterinary Internal Medicine. 2018.
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abstract = "Background: Gastroesophageal reflux and microaspiration (MA) of gastric juice are associated with various human respiratory diseases but not in dogs. Objective: To detect the presence of bile acids in bronchoalveolar lavage fluid (BALF) of dogs with various respiratory diseases. Animals: Twenty-seven West Highland White Terriers (WHWTs) with canine idiopathic pulmonary fibrosis (CIPF), 11 dogs with bacterial pneumonia (BP), 13 with chronic bronchitis (CB), 9 with eosinophilic bronchopneumopathy (EBP), 19 with laryngeal dysfunction (LD), 8 Irish Wolfhounds (IWHs) with previous BPs, 13 healthy WHWTs, all privately owned dogs, and 6 healthy research colony Beagles. Methods: Prospective cross-sectional observational study with convenience sampling of dogs. Bile acids were measured by mass spectrometry in BALF samples. Total bile acid (TBA) concentration was calculated as a sum of 17 different bile acids. Results: Concentrations of TBA were above the limit of quantification in 78{\%} of CIPF, 45{\%} of BP, 62{\%} of CB, 44{\%} of EBP, 68{\%} of LD, and 13{\%} of IWH dogs. In healthy dogs, bile acids were detected less commonly in Beagles (0/6) than in healthy WHWTs (10/13). Concentrations of TBA were significantly higher in CIPF (median 0.013 μM, range not quantifiable [n.q.]-0.14 μM, P <.001), healthy WHWTs (0.0052 μM, n.q.-1.2 μM, P =.003), LD (0.010 μM, n.q.-2.3 μM, P =.015), and CB (0.0078 μM, n.q.-0.073 μM, P =.018) groups compared to Beagles (0 μM, n.q.). Conclusion and Clinical Importance: These results suggest that MA occurs in various respiratory diseases of dogs and also in healthy WHWTs.",
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AU - Lilja-Maula, L.

AU - Melamies, M.

AU - Viitanen, S. J.

AU - Johnson, Lynelle R

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AU - Niemi, M.

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