Reduction of EphA4 receptor expression after spinal cord injury does not induce axonal regeneration or return of tcMMEP response

Lillian Cruz-Orengo, Johnny D. Figueroa, Aranza Torrado, Anabel Puig, Scott R. Whittemore, Jorge D. Miranda

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Spinal cord injury (SCI) causes an increase of inhibitory factors that may restrict axonal outgrowth after trauma. During the past decade, the Eph receptors and ephrin ligands have emerged as key repulsive cues known to be involved in neurite outgrowth, synapse formation, and axonal pathfinding during development. Given the non-permissive environment for axonal regeneration after SCI, we questioned whether enhanced-expression of the EphA4 receptor with repulsive activity for axonal outgrowth is potentially responsible for the regenerative failure. To address this possibility, we have examined the expression of EphA4 after SCI in adult rats following a contusion SCI. EphA4 expression studies demonstrated a time-dependent change for EphA4 protein without alterations in β-actin. EphA4 was downregulated initially and upregulated 7 days after injury. Blockade of EphA4 upregulation with antisense oligonucleotides did not produce an anatomical or physiological response monitored with anterograde tracing studies or transcranial magnetic motor evoked potentials (tcMMEP), respectively. These results demonstrated that upregulation of EphA4 receptors after trauma is not related to axonal regeneration or return of nerve conduction across the injury site.

Original languageEnglish (US)
Pages (from-to)49-54
Number of pages6
JournalNeuroscience Letters
Volume418
Issue number1
DOIs
StatePublished - May 11 2007
Externally publishedYes

Fingerprint

EphA4 Receptor
Motor Evoked Potentials
Spinal Cord Injuries
Regeneration
Ephrins
Wounds and Injuries
Up-Regulation
Contusions
Antisense Oligonucleotides
Neural Conduction
Synapses
Cues
Actins
Down-Regulation

Keywords

  • EphA
  • Ephrin
  • Regeneration
  • Sprouting
  • Tracing
  • Trauma

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Reduction of EphA4 receptor expression after spinal cord injury does not induce axonal regeneration or return of tcMMEP response. / Cruz-Orengo, Lillian; Figueroa, Johnny D.; Torrado, Aranza; Puig, Anabel; Whittemore, Scott R.; Miranda, Jorge D.

In: Neuroscience Letters, Vol. 418, No. 1, 11.05.2007, p. 49-54.

Research output: Contribution to journalArticle

Cruz-Orengo, Lillian ; Figueroa, Johnny D. ; Torrado, Aranza ; Puig, Anabel ; Whittemore, Scott R. ; Miranda, Jorge D. / Reduction of EphA4 receptor expression after spinal cord injury does not induce axonal regeneration or return of tcMMEP response. In: Neuroscience Letters. 2007 ; Vol. 418, No. 1. pp. 49-54.
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