Reduction of bleomycin-induced sequestration of neutrophils and vascular injury in hamster lungs by nitroglycerin

Q. Wang, D. R. Haynam, D. M. Hyde, A. B. Combs, S. N. Giri

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Neutrophil sequestration within the pulmonary microvasculature contributes to the pathogenesis of various types of lung injury. The present study was designed to test the hypothesis that nitroglycerin, a vasodilator, could decrease neutrophil sequestration within the hamster lung, and thus lead to reduction of bleomycin-induced lung inflammation and injury. Nitroglycerin in an ointment base or petroleum jelly was dermally applied twice daily to the backs of hamsters which were given a single dose of bleomycin or 0.9% NaCl intratracheally. Nitroglycerin significantly reduced the bleomycin-induced increase in neutrophil sequestration in the lungs as indicated by a decrease in the number of neutrophils in bronchoalveolar lavage fluid (BALF), and by both BALF and tissue myeloperoxidase activity. Similarly, nitroglycerin significantly attenuated bleomycin-induced increases in protein content and acid phosphatase activity in the BALF supernatant. There was a marginal, but nonstatistically significant, reduction of bleomycin-induced increases in lung hydroxyproline content and malondialdehyde production by nitroglycerin. It was concluded that nitroglycerin, acting presumably by inducing pulmonary vasodilation, caused a marked reduction in the bleomycin-induced neutrophil sequestration and thus offered protection against the vascular leakage and lung injury in the model.

Original languageEnglish (US)
Pages (from-to)249-261
Number of pages13
JournalInflammopharmacology
Volume1
Issue number3
DOIs
StatePublished - Sep 1992

Keywords

  • bleomycin
  • hamster lung
  • myeloperoxidase
  • neutrophils
  • nitroglycerin

ASJC Scopus subject areas

  • Pharmacology
  • Immunology
  • Pharmacology (medical)

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