Reduction in wound bioburden using a silver-loaded dissolvable microfilm construct

Maggie Herron, Ankit Agarwal, Patricia R. Kierski, Diego F. Calderon, Leandro B C Teixeira, Michael J. Schurr, Christopher J Murphy, Charles J. Czuprynski, Jonathan F. Mcanulty, Nicholas L. Abbott

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Silver is a widely used antimicrobial agent, yet, when impregnated in macroscopic dressings, it stains wounds, can lead to tissue toxicity, and can inhibit healing. Recently, polymeric nanofilms containing silver nanoparticles were reported to exhibit antimicrobial activity at loadings and release rates of silver that are 100× lower than conventional dressings. Here, fabrication of composite microfilm constructs that provide a facile way to transfer the silver-loaded polymeric nanofilms onto wounds in vivo is reported. The construct is fabricated from a silver nanoparticle-loaded polymeric nanofilm that is laminated with a micrometer-thick-soluble film of polyvinylalcohol (PVA). When placed on a moist wound, the PVA dissolves, leaving the silver-loaded nanofilm immobilized on the wound-bed. In vitro, the immobilized nanofilms release <1 μg cm-2 d-1 of silver over 30 d from skin dermis and they kill 5 log10 CFUs of Staphylococcus aureus in 24 h. In mice, wounds inoculated with 105 CFU S. aureus presented up to 3 log10 less bacterial burden when treated with silver/nanofilms for 3 d, as compared to unmodified wounds. In uncontaminated wounds, silver/nanofilms allow normal and complete wound closure by re-epithelialization. Dissolvable microfilm constructs may overcome key limitations associated with current uses of silver in wound healing.

Original languageEnglish (US)
Pages (from-to)916-928
Number of pages13
JournalAdvanced healthcare materials
Volume3
Issue number6
DOIs
StatePublished - 2014

Fingerprint

Microfilm
Silver
Wounds and Injuries
Bandages
Nanoparticles
Staphylococcus aureus
Re-Epithelialization
Antimicrobial agents
Dermis
Anti-Infective Agents
Thick films
Wound Healing
Toxicity
Skin
Coloring Agents

Keywords

  • Bacteria
  • Microfilms
  • Polymers
  • Silver
  • Wound healing

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Pharmaceutical Science
  • Medicine(all)

Cite this

Herron, M., Agarwal, A., Kierski, P. R., Calderon, D. F., Teixeira, L. B. C., Schurr, M. J., ... Abbott, N. L. (2014). Reduction in wound bioburden using a silver-loaded dissolvable microfilm construct. Advanced healthcare materials, 3(6), 916-928. https://doi.org/10.1002/adhm.201300537

Reduction in wound bioburden using a silver-loaded dissolvable microfilm construct. / Herron, Maggie; Agarwal, Ankit; Kierski, Patricia R.; Calderon, Diego F.; Teixeira, Leandro B C; Schurr, Michael J.; Murphy, Christopher J; Czuprynski, Charles J.; Mcanulty, Jonathan F.; Abbott, Nicholas L.

In: Advanced healthcare materials, Vol. 3, No. 6, 2014, p. 916-928.

Research output: Contribution to journalArticle

Herron, M, Agarwal, A, Kierski, PR, Calderon, DF, Teixeira, LBC, Schurr, MJ, Murphy, CJ, Czuprynski, CJ, Mcanulty, JF & Abbott, NL 2014, 'Reduction in wound bioburden using a silver-loaded dissolvable microfilm construct', Advanced healthcare materials, vol. 3, no. 6, pp. 916-928. https://doi.org/10.1002/adhm.201300537
Herron M, Agarwal A, Kierski PR, Calderon DF, Teixeira LBC, Schurr MJ et al. Reduction in wound bioburden using a silver-loaded dissolvable microfilm construct. Advanced healthcare materials. 2014;3(6):916-928. https://doi.org/10.1002/adhm.201300537
Herron, Maggie ; Agarwal, Ankit ; Kierski, Patricia R. ; Calderon, Diego F. ; Teixeira, Leandro B C ; Schurr, Michael J. ; Murphy, Christopher J ; Czuprynski, Charles J. ; Mcanulty, Jonathan F. ; Abbott, Nicholas L. / Reduction in wound bioburden using a silver-loaded dissolvable microfilm construct. In: Advanced healthcare materials. 2014 ; Vol. 3, No. 6. pp. 916-928.
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