Abstract
Silver is a widely used antimicrobial agent, yet, when impregnated in macroscopic dressings, it stains wounds, can lead to tissue toxicity, and can inhibit healing. Recently, polymeric nanofilms containing silver nanoparticles were reported to exhibit antimicrobial activity at loadings and release rates of silver that are 100× lower than conventional dressings. Here, fabrication of composite microfilm constructs that provide a facile way to transfer the silver-loaded polymeric nanofilms onto wounds in vivo is reported. The construct is fabricated from a silver nanoparticle-loaded polymeric nanofilm that is laminated with a micrometer-thick-soluble film of polyvinylalcohol (PVA). When placed on a moist wound, the PVA dissolves, leaving the silver-loaded nanofilm immobilized on the wound-bed. In vitro, the immobilized nanofilms release <1 μg cm-2 d-1 of silver over 30 d from skin dermis and they kill 5 log10 CFUs of Staphylococcus aureus in 24 h. In mice, wounds inoculated with 105 CFU S. aureus presented up to 3 log10 less bacterial burden when treated with silver/nanofilms for 3 d, as compared to unmodified wounds. In uncontaminated wounds, silver/nanofilms allow normal and complete wound closure by re-epithelialization. Dissolvable microfilm constructs may overcome key limitations associated with current uses of silver in wound healing.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 916-928 |
| Number of pages | 13 |
| Journal | Advanced healthcare materials |
| Volume | 3 |
| Issue number | 6 |
| DOIs | |
| State | Published - 2014 |
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Keywords
- Bacteria
- Microfilms
- Polymers
- Silver
- Wound healing
ASJC Scopus subject areas
- Biomedical Engineering
- Biomaterials
- Pharmaceutical Science
- Medicine(all)
Cite this
Reduction in wound bioburden using a silver-loaded dissolvable microfilm construct. / Herron, Maggie; Agarwal, Ankit; Kierski, Patricia R.; Calderon, Diego F.; Teixeira, Leandro B C; Schurr, Michael J.; Murphy, Christopher J; Czuprynski, Charles J.; Mcanulty, Jonathan F.; Abbott, Nicholas L.
In: Advanced healthcare materials, Vol. 3, No. 6, 2014, p. 916-928.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Reduction in wound bioburden using a silver-loaded dissolvable microfilm construct
AU - Herron, Maggie
AU - Agarwal, Ankit
AU - Kierski, Patricia R.
AU - Calderon, Diego F.
AU - Teixeira, Leandro B C
AU - Schurr, Michael J.
AU - Murphy, Christopher J
AU - Czuprynski, Charles J.
AU - Mcanulty, Jonathan F.
AU - Abbott, Nicholas L.
PY - 2014
Y1 - 2014
N2 - Silver is a widely used antimicrobial agent, yet, when impregnated in macroscopic dressings, it stains wounds, can lead to tissue toxicity, and can inhibit healing. Recently, polymeric nanofilms containing silver nanoparticles were reported to exhibit antimicrobial activity at loadings and release rates of silver that are 100× lower than conventional dressings. Here, fabrication of composite microfilm constructs that provide a facile way to transfer the silver-loaded polymeric nanofilms onto wounds in vivo is reported. The construct is fabricated from a silver nanoparticle-loaded polymeric nanofilm that is laminated with a micrometer-thick-soluble film of polyvinylalcohol (PVA). When placed on a moist wound, the PVA dissolves, leaving the silver-loaded nanofilm immobilized on the wound-bed. In vitro, the immobilized nanofilms release <1 μg cm-2 d-1 of silver over 30 d from skin dermis and they kill 5 log10 CFUs of Staphylococcus aureus in 24 h. In mice, wounds inoculated with 105 CFU S. aureus presented up to 3 log10 less bacterial burden when treated with silver/nanofilms for 3 d, as compared to unmodified wounds. In uncontaminated wounds, silver/nanofilms allow normal and complete wound closure by re-epithelialization. Dissolvable microfilm constructs may overcome key limitations associated with current uses of silver in wound healing.
AB - Silver is a widely used antimicrobial agent, yet, when impregnated in macroscopic dressings, it stains wounds, can lead to tissue toxicity, and can inhibit healing. Recently, polymeric nanofilms containing silver nanoparticles were reported to exhibit antimicrobial activity at loadings and release rates of silver that are 100× lower than conventional dressings. Here, fabrication of composite microfilm constructs that provide a facile way to transfer the silver-loaded polymeric nanofilms onto wounds in vivo is reported. The construct is fabricated from a silver nanoparticle-loaded polymeric nanofilm that is laminated with a micrometer-thick-soluble film of polyvinylalcohol (PVA). When placed on a moist wound, the PVA dissolves, leaving the silver-loaded nanofilm immobilized on the wound-bed. In vitro, the immobilized nanofilms release <1 μg cm-2 d-1 of silver over 30 d from skin dermis and they kill 5 log10 CFUs of Staphylococcus aureus in 24 h. In mice, wounds inoculated with 105 CFU S. aureus presented up to 3 log10 less bacterial burden when treated with silver/nanofilms for 3 d, as compared to unmodified wounds. In uncontaminated wounds, silver/nanofilms allow normal and complete wound closure by re-epithelialization. Dissolvable microfilm constructs may overcome key limitations associated with current uses of silver in wound healing.
KW - Bacteria
KW - Microfilms
KW - Polymers
KW - Silver
KW - Wound healing
UR - http://www.scopus.com/inward/record.url?scp=84902295680&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84902295680&partnerID=8YFLogxK
U2 - 10.1002/adhm.201300537
DO - 10.1002/adhm.201300537
M3 - Article
C2 - 24523027
AN - SCOPUS:84902295680
VL - 3
SP - 916
EP - 928
JO - Advanced healthcare materials
JF - Advanced healthcare materials
SN - 2192-2640
IS - 6
ER -