Reducing estrogen synthesis in developing boars increases testis size and total sperm production

Eeman E. At-Taras, Trish Berger, Megan J. McCarthy, Alan J Conley, Barbara J. Nitta-Oda, Janet F. Roser

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The abundant production of testicular estrogens and the presence of both ESR1 and ESR2 within boar testes are consistent with a role for estrogen in testicular development and/or function in this species. This study was aimed at determining the role of endogenous estrogen in the regulation of testicular development and function, including the effects on testis weight, histology, sperm production (detergent-resistant spermatid numbers), Sertoli cell numbers, and Leydig cell volume in the boar. Twenty-eight littermate pairs of boars were assigned to groups as follows: 1 boar from each pair was assigned to the control group (vehicle) and the other was assigned to treatment and received 0.1 mg/kg body weight of an aromatase enzyme inhibitor (letrozole) orally each week beginning at 1 week of age until castration at 2, 3, 4, 5, 6, 7, or 8 months of age. Testes were weighed and testicular parenchyma was recovered for determination of histology and detergent-resistant spermatid numbers, and for determination of Sertoli cell number and Leydig cell volume by staining for GATA-4 and 17-α hydroxylase/17-20 lyase respectively. Testes of aromatase-inhibited boars initially exhibited delayed lumen formation, lower testicular weight, fewer detergent-resistant spermatids, and fewer Sertoli cells, but by 7 to 8 months, these boars had recovered and had larger testes, more detergent-resistant spermatids per testis, and more Sertoli cells. Total Leydig cell volume increased in proportion to testis size. Reducing endogenous estrogen is consistent with a delay in testicular maturation/puberty that allows for a longer window for the proliferation of Sertoli cells and maturation of Leydig cells, resulting in larger testes and higher spermatid production.

Original languageEnglish (US)
Pages (from-to)552-559
Number of pages8
JournalJournal of Andrology
Volume27
Issue number4
DOIs
StatePublished - Jul 2006

Fingerprint

Spermatozoa
Testis
Estrogens
Spermatids
Sertoli Cells
Leydig Cells
Detergents
Cell Size
letrozole
Histology
Cell Count
Steroid 17-alpha-Hydroxylase
Weights and Measures
Aromatase Inhibitors
Aromatase
Castration
Enzyme Inhibitors
Puberty
Mixed Function Oxygenases
Body Weight

Keywords

  • Aromatase inhibition
  • Leydig cell
  • Sertoli cell

ASJC Scopus subject areas

  • Endocrinology

Cite this

Reducing estrogen synthesis in developing boars increases testis size and total sperm production. / At-Taras, Eeman E.; Berger, Trish; McCarthy, Megan J.; Conley, Alan J; Nitta-Oda, Barbara J.; Roser, Janet F.

In: Journal of Andrology, Vol. 27, No. 4, 07.2006, p. 552-559.

Research output: Contribution to journalArticle

At-Taras, Eeman E. ; Berger, Trish ; McCarthy, Megan J. ; Conley, Alan J ; Nitta-Oda, Barbara J. ; Roser, Janet F. / Reducing estrogen synthesis in developing boars increases testis size and total sperm production. In: Journal of Andrology. 2006 ; Vol. 27, No. 4. pp. 552-559.
@article{8cbd87b97111438d9818b9bc76b425f8,
title = "Reducing estrogen synthesis in developing boars increases testis size and total sperm production",
abstract = "The abundant production of testicular estrogens and the presence of both ESR1 and ESR2 within boar testes are consistent with a role for estrogen in testicular development and/or function in this species. This study was aimed at determining the role of endogenous estrogen in the regulation of testicular development and function, including the effects on testis weight, histology, sperm production (detergent-resistant spermatid numbers), Sertoli cell numbers, and Leydig cell volume in the boar. Twenty-eight littermate pairs of boars were assigned to groups as follows: 1 boar from each pair was assigned to the control group (vehicle) and the other was assigned to treatment and received 0.1 mg/kg body weight of an aromatase enzyme inhibitor (letrozole) orally each week beginning at 1 week of age until castration at 2, 3, 4, 5, 6, 7, or 8 months of age. Testes were weighed and testicular parenchyma was recovered for determination of histology and detergent-resistant spermatid numbers, and for determination of Sertoli cell number and Leydig cell volume by staining for GATA-4 and 17-α hydroxylase/17-20 lyase respectively. Testes of aromatase-inhibited boars initially exhibited delayed lumen formation, lower testicular weight, fewer detergent-resistant spermatids, and fewer Sertoli cells, but by 7 to 8 months, these boars had recovered and had larger testes, more detergent-resistant spermatids per testis, and more Sertoli cells. Total Leydig cell volume increased in proportion to testis size. Reducing endogenous estrogen is consistent with a delay in testicular maturation/puberty that allows for a longer window for the proliferation of Sertoli cells and maturation of Leydig cells, resulting in larger testes and higher spermatid production.",
keywords = "Aromatase inhibition, Leydig cell, Sertoli cell",
author = "At-Taras, {Eeman E.} and Trish Berger and McCarthy, {Megan J.} and Conley, {Alan J} and Nitta-Oda, {Barbara J.} and Roser, {Janet F.}",
year = "2006",
month = "7",
doi = "10.2164/jandrol.05195",
language = "English (US)",
volume = "27",
pages = "552--559",
journal = "Journal of Andrology",
issn = "0196-3635",
publisher = "American Society of Andrology Inc.",
number = "4",

}

TY - JOUR

T1 - Reducing estrogen synthesis in developing boars increases testis size and total sperm production

AU - At-Taras, Eeman E.

AU - Berger, Trish

AU - McCarthy, Megan J.

AU - Conley, Alan J

AU - Nitta-Oda, Barbara J.

AU - Roser, Janet F.

PY - 2006/7

Y1 - 2006/7

N2 - The abundant production of testicular estrogens and the presence of both ESR1 and ESR2 within boar testes are consistent with a role for estrogen in testicular development and/or function in this species. This study was aimed at determining the role of endogenous estrogen in the regulation of testicular development and function, including the effects on testis weight, histology, sperm production (detergent-resistant spermatid numbers), Sertoli cell numbers, and Leydig cell volume in the boar. Twenty-eight littermate pairs of boars were assigned to groups as follows: 1 boar from each pair was assigned to the control group (vehicle) and the other was assigned to treatment and received 0.1 mg/kg body weight of an aromatase enzyme inhibitor (letrozole) orally each week beginning at 1 week of age until castration at 2, 3, 4, 5, 6, 7, or 8 months of age. Testes were weighed and testicular parenchyma was recovered for determination of histology and detergent-resistant spermatid numbers, and for determination of Sertoli cell number and Leydig cell volume by staining for GATA-4 and 17-α hydroxylase/17-20 lyase respectively. Testes of aromatase-inhibited boars initially exhibited delayed lumen formation, lower testicular weight, fewer detergent-resistant spermatids, and fewer Sertoli cells, but by 7 to 8 months, these boars had recovered and had larger testes, more detergent-resistant spermatids per testis, and more Sertoli cells. Total Leydig cell volume increased in proportion to testis size. Reducing endogenous estrogen is consistent with a delay in testicular maturation/puberty that allows for a longer window for the proliferation of Sertoli cells and maturation of Leydig cells, resulting in larger testes and higher spermatid production.

AB - The abundant production of testicular estrogens and the presence of both ESR1 and ESR2 within boar testes are consistent with a role for estrogen in testicular development and/or function in this species. This study was aimed at determining the role of endogenous estrogen in the regulation of testicular development and function, including the effects on testis weight, histology, sperm production (detergent-resistant spermatid numbers), Sertoli cell numbers, and Leydig cell volume in the boar. Twenty-eight littermate pairs of boars were assigned to groups as follows: 1 boar from each pair was assigned to the control group (vehicle) and the other was assigned to treatment and received 0.1 mg/kg body weight of an aromatase enzyme inhibitor (letrozole) orally each week beginning at 1 week of age until castration at 2, 3, 4, 5, 6, 7, or 8 months of age. Testes were weighed and testicular parenchyma was recovered for determination of histology and detergent-resistant spermatid numbers, and for determination of Sertoli cell number and Leydig cell volume by staining for GATA-4 and 17-α hydroxylase/17-20 lyase respectively. Testes of aromatase-inhibited boars initially exhibited delayed lumen formation, lower testicular weight, fewer detergent-resistant spermatids, and fewer Sertoli cells, but by 7 to 8 months, these boars had recovered and had larger testes, more detergent-resistant spermatids per testis, and more Sertoli cells. Total Leydig cell volume increased in proportion to testis size. Reducing endogenous estrogen is consistent with a delay in testicular maturation/puberty that allows for a longer window for the proliferation of Sertoli cells and maturation of Leydig cells, resulting in larger testes and higher spermatid production.

KW - Aromatase inhibition

KW - Leydig cell

KW - Sertoli cell

UR - http://www.scopus.com/inward/record.url?scp=33746435317&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33746435317&partnerID=8YFLogxK

U2 - 10.2164/jandrol.05195

DO - 10.2164/jandrol.05195

M3 - Article

VL - 27

SP - 552

EP - 559

JO - Journal of Andrology

JF - Journal of Andrology

SN - 0196-3635

IS - 4

ER -