Reduced spinal cord sensitization to C-fibre stimulation in mice over-expressing galanin

Stefan Grass, Jacqueline Crawley, Xiao Jun Xu, Zsuzsanna Wiesenfeld-Hallin

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

The neuropeptide galanin may have a role in spinal nociception. In this study, we examined the excitability of the flexor reflex and its sensitization by repetitive stimulation of nociceptive C-fibres in anaesthetized mice that over-express galanin. No difference was seen between over-expressing galanin and wild-type mice in the magnitude of the baseline flexor reflex. Repetitive conditioning stimulation of C-fibres (10 stimuli at 1 Hz) produced a gradual increase in reflex magnitude during the conditioning stimulation (wind-up), as well as an increase in spinal reflex excitability after the termination of the stimulus train (central sensitization) in wild-type mice. Although the windup did not differ between over-expressing galanin and wild-type mice, the magnitude of central sensitization was significantly reduced in the over-expressing galanin mice (24 ± 13% peak increase compared with 164 ± 65% in the wild-type). Intrathecal administration of M35, a galanin receptor antagonist, markedly enhanced central sensitization in over-expressing galanin mice in association with C-fibre conditioning stimulation, while having no effect in wild-type mice. These results provide further electrophysiological evidence for an inhibitory function of galanin in modulation of central sensitization in response to C-fibre stimulation.

Original languageEnglish (US)
Pages (from-to)1829-1832
Number of pages4
JournalEuropean Journal of Neuroscience
Volume17
Issue number9
DOIs
StatePublished - May 2003
Externally publishedYes

Keywords

  • Central sensitization
  • Flexor reflex
  • Nociception
  • Pain
  • Transgenic mice
  • Wind-up

ASJC Scopus subject areas

  • Neuroscience(all)

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