Reduced recombination rate and genetic differentiation between the M and S forms of Anopheles gambiae s.s.

Michel A. Slotman, Lisa J. Reimer, Tara Thiemann, Guimogo Dolo, Etienne Fondjo, Gregory C Lanzaro

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Genetic differentiation between the largely sympatric molecular forms M and S of Anopheles gambiae appears mostly limited to division 6 and part of division 5 of the X chromosome. This region is adjacent to the centromere and includes the rDNA that was used to define these forms. This localized differentiation between populations that experience gene flow strongly suggests that this region contains genes responsible for reproductive isolation. Regions adjacent to centromeres are known to experience less recombination in several species and it has recently been suggested that low recombination rates can facilitate the accumulation and maintenance of isolation genes in partially isolated populations. Therefore, we measured the recombination rate in division 5D/6 directly and estimate that it is at least 16-fold reduced across this region compared to the remainder of the X chromosome. Additionally, sequence data from four loci from field-collected mosquitoes from several West African countries show very strong differentiation between the molecular forms in division 5D/6, whereas none was observed in two loci elsewhere on the X chromosome. Furthermore, genetic variation was substantially lower in division 5D/6 compared to the two reference loci, and the inferred genealogies of the division 5D/6 genes show patterns consistent with selective sweeps. This suggests that the reduced recombination rate has increased the effect of selection on this region and that our data are consistent with the hypothesis that reduced recombination rates can play a role in the accumulation of isolation genes in the face of gene flow.

Original languageEnglish (US)
Pages (from-to)2081-2093
Number of pages13
JournalGenetics
Volume174
Issue number4
DOIs
StatePublished - 2006

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Anopheles gambiae
Genetic Recombination
X Chromosome
Gene Flow
Centromere
Genes
Reproductive Isolation
Genealogy and Heraldry
Ribosomal DNA
Culicidae
Population
Maintenance

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Reduced recombination rate and genetic differentiation between the M and S forms of Anopheles gambiae s.s. / Slotman, Michel A.; Reimer, Lisa J.; Thiemann, Tara; Dolo, Guimogo; Fondjo, Etienne; Lanzaro, Gregory C.

In: Genetics, Vol. 174, No. 4, 2006, p. 2081-2093.

Research output: Contribution to journalArticle

Slotman, Michel A. ; Reimer, Lisa J. ; Thiemann, Tara ; Dolo, Guimogo ; Fondjo, Etienne ; Lanzaro, Gregory C. / Reduced recombination rate and genetic differentiation between the M and S forms of Anopheles gambiae s.s. In: Genetics. 2006 ; Vol. 174, No. 4. pp. 2081-2093.
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