Reduced hepatic expression of glutathione S-transferases in burned mice

Tam N. Pham, Kiho Cho, Aimee J. Warren, David G Greenhalgh

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Background: Glutathione S-transferases (GSTs) contribute to liver homeostasis and are released into plasma after liver injury. This study investigates the hepatic expression of key GST enzyme subtypes after burn injury. Methods: Mice were subjected to an 18% total body surface area burn. Expression of mGSTα3 and mGSTμ1 was analyzed by reverse-transcriptase polymerase chain reaction and Western blots on liver preparations from burned and control mice. GST catalytic activity was measured by conjugation assays. Results: In burned animals, mGSTα3 mRNA was only reduced at 1 day, whereas mGSTμ1 mRNA decreased at 1 day and at 3 days after injury. Total mGSTμ, protein levels decreased at the same time points, whereas total mGSTα protein levels did not change. Also, by 3 days, total GST conjugation activity was reduced by 25% (p = 0.05). Conclusion: Reduced expression of specific α and μ GST enzymes after burns correlates functionally with reduced GST activity. Altered GST expression may contribute to liver damage after systemic injury.

Original languageEnglish (US)
Pages (from-to)867-871
Number of pages5
JournalJournal of Trauma - Injury, Infection and Critical Care
Volume57
Issue number4
DOIs
StatePublished - Oct 2004

Keywords

  • Burn injury
  • Glutathione S-transferase activity
  • Liver
  • Metabolism
  • Transcription

ASJC Scopus subject areas

  • Surgery

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