Reduced excitatory neurotransmission and mild Autism-Relevant phenotypes in adolescent shank3 null mutant mice

Mu Yang, Ozlem Bozdagi, Maria Luisa Scattoni, Markus Wöhr, Florence I. Roullet, Adam M. Katz, Danielle N. Abrams, David Kalikhman, Harrison Simon, Leuk Woldeyohannes, James Y. Zhang, Mark J. Harris, Roheeni Saxena, Jill L Silverman, Joseph D. Buxbaum, Jacqueline Crawley

Research output: Contribution to journalArticle

180 Citations (Scopus)

Abstract

Mutations in the synaptic scaffolding protein gene SHANK3 are strongly implicated in autism and Phelan-McDermid 22q13 deletion syndrome. The precise location of the mutation within the Shank3 gene is key to its phenotypic outcomes. Here, we report the physiological and behavioral consequences of null and heterozygous mutations in the ankyrin repeat domaininShank3mice. Both homozygous and heterozygous mice showed reduced glutamatergic transmission and long-term potentiation in the hippocampus with more severe deficits detected in the homozygous mice. Three independent cohorts were evaluated for magnitude and replicability of behavioral endopheno types relevant to autism and Phelan-McDermid syndrome. Mild social impairments were detected, primarily in juveniles during reciprocal interactions, while all genotypes displayed normal adult sociability on the three-chambered task. Impaired novel object recognition and rotarod performance were consistent across cohorts of null mutants. Repetitive self-grooming, reduced ultrasonic vocalizations, and deficits in reversal of water maze learning were detected only in some cohorts, emphasizing the importance of replication analyses. These results demonstrate the exquisite specificity of deletions in discrete domains within the Shank3 gene in determining severity of symptoms.

Original languageEnglish (US)
Pages (from-to)6525-6541
Number of pages17
JournalJournal of Neuroscience
Volume32
Issue number19
DOIs
StatePublished - May 9 2012
Externally publishedYes

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Autistic Disorder
Synaptic Transmission
Phenotype
Mutation
Maze Learning
Ankyrin Repeat
Grooming
Long-Term Potentiation
Ultrasonics
Genes
Hippocampus
Genotype
Water
Proteins
Telomeric 22q13 Monosomy Syndrome

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Reduced excitatory neurotransmission and mild Autism-Relevant phenotypes in adolescent shank3 null mutant mice. / Yang, Mu; Bozdagi, Ozlem; Scattoni, Maria Luisa; Wöhr, Markus; Roullet, Florence I.; Katz, Adam M.; Abrams, Danielle N.; Kalikhman, David; Simon, Harrison; Woldeyohannes, Leuk; Zhang, James Y.; Harris, Mark J.; Saxena, Roheeni; Silverman, Jill L; Buxbaum, Joseph D.; Crawley, Jacqueline.

In: Journal of Neuroscience, Vol. 32, No. 19, 09.05.2012, p. 6525-6541.

Research output: Contribution to journalArticle

Yang, M, Bozdagi, O, Scattoni, ML, Wöhr, M, Roullet, FI, Katz, AM, Abrams, DN, Kalikhman, D, Simon, H, Woldeyohannes, L, Zhang, JY, Harris, MJ, Saxena, R, Silverman, JL, Buxbaum, JD & Crawley, J 2012, 'Reduced excitatory neurotransmission and mild Autism-Relevant phenotypes in adolescent shank3 null mutant mice', Journal of Neuroscience, vol. 32, no. 19, pp. 6525-6541. https://doi.org/10.1523/JNEUROSCI.6107-11.2012
Yang, Mu ; Bozdagi, Ozlem ; Scattoni, Maria Luisa ; Wöhr, Markus ; Roullet, Florence I. ; Katz, Adam M. ; Abrams, Danielle N. ; Kalikhman, David ; Simon, Harrison ; Woldeyohannes, Leuk ; Zhang, James Y. ; Harris, Mark J. ; Saxena, Roheeni ; Silverman, Jill L ; Buxbaum, Joseph D. ; Crawley, Jacqueline. / Reduced excitatory neurotransmission and mild Autism-Relevant phenotypes in adolescent shank3 null mutant mice. In: Journal of Neuroscience. 2012 ; Vol. 32, No. 19. pp. 6525-6541.
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