Abstract
Environmental enrichment results in increased levels of Fmrp in brain and increased dendritic complexity. The present experiment evaluated activity-dependent increases in Fmrp levels in the motor cortex in response to training on a skilled forelimb reaching task in the CGG KI mouse model of the fragile X premutation. Fmrp, Arc, and c-Fos protein levels were quantified by Western blot in the contralateral motor cortex of mice following training to reach for sucrose pellets with a non-preferred paw and compared to levels in the ipsilateral motor cortex. After training, all mice showed increases in Fmrp, Arc, and c-Fos protein levels in the contralateral compared to the ipsilateral hemisphere; however, the increase in CGG KI mice was less than wildtype mice. Increases in Fmrp and Arc proteins scaled with learning, whereas this relationship was not observed with the c-Fos levels. These data suggest the possibility that reduced levels of activity-dependent proteins associated with synaptic plasticity such as Fmrp and Arc may contribute to the neurocognitive phenotype reported in the CGG KI mice and the fragile X premutation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 160-168 |
| Number of pages | 9 |
| Journal | Neurobiology of Learning and Memory |
| Volume | 109 |
| DOIs | |
| State | Published - Mar 2014 |
Fingerprint
Keywords
- Arc
- C-Fos
- CGG KI mouse
- Endophenotype
- Fmrp
- Fragile X premutation
- Western blot
ASJC Scopus subject areas
- Behavioral Neuroscience
- Cognitive Neuroscience
- Experimental and Cognitive Psychology
Cite this
Reduced activity-dependent protein levels in a mouse model of the fragile X premutation. / von Leden, Ramona E.; Curley, Lindsey C.; Greenberg, Gian D.; Hunsaker, Michael R.; Willemsen, Rob; Berman, Robert F.
In: Neurobiology of Learning and Memory, Vol. 109, 03.2014, p. 160-168.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Reduced activity-dependent protein levels in a mouse model of the fragile X premutation
AU - von Leden, Ramona E.
AU - Curley, Lindsey C.
AU - Greenberg, Gian D.
AU - Hunsaker, Michael R.
AU - Willemsen, Rob
AU - Berman, Robert F
PY - 2014/3
Y1 - 2014/3
N2 - Environmental enrichment results in increased levels of Fmrp in brain and increased dendritic complexity. The present experiment evaluated activity-dependent increases in Fmrp levels in the motor cortex in response to training on a skilled forelimb reaching task in the CGG KI mouse model of the fragile X premutation. Fmrp, Arc, and c-Fos protein levels were quantified by Western blot in the contralateral motor cortex of mice following training to reach for sucrose pellets with a non-preferred paw and compared to levels in the ipsilateral motor cortex. After training, all mice showed increases in Fmrp, Arc, and c-Fos protein levels in the contralateral compared to the ipsilateral hemisphere; however, the increase in CGG KI mice was less than wildtype mice. Increases in Fmrp and Arc proteins scaled with learning, whereas this relationship was not observed with the c-Fos levels. These data suggest the possibility that reduced levels of activity-dependent proteins associated with synaptic plasticity such as Fmrp and Arc may contribute to the neurocognitive phenotype reported in the CGG KI mice and the fragile X premutation.
AB - Environmental enrichment results in increased levels of Fmrp in brain and increased dendritic complexity. The present experiment evaluated activity-dependent increases in Fmrp levels in the motor cortex in response to training on a skilled forelimb reaching task in the CGG KI mouse model of the fragile X premutation. Fmrp, Arc, and c-Fos protein levels were quantified by Western blot in the contralateral motor cortex of mice following training to reach for sucrose pellets with a non-preferred paw and compared to levels in the ipsilateral motor cortex. After training, all mice showed increases in Fmrp, Arc, and c-Fos protein levels in the contralateral compared to the ipsilateral hemisphere; however, the increase in CGG KI mice was less than wildtype mice. Increases in Fmrp and Arc proteins scaled with learning, whereas this relationship was not observed with the c-Fos levels. These data suggest the possibility that reduced levels of activity-dependent proteins associated with synaptic plasticity such as Fmrp and Arc may contribute to the neurocognitive phenotype reported in the CGG KI mice and the fragile X premutation.
KW - Arc
KW - C-Fos
KW - CGG KI mouse
KW - Endophenotype
KW - Fmrp
KW - Fragile X premutation
KW - Western blot
UR - http://www.scopus.com/inward/record.url?scp=84893555489&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84893555489&partnerID=8YFLogxK
U2 - 10.1016/j.nlm.2014.01.011
DO - 10.1016/j.nlm.2014.01.011
M3 - Article
C2 - 24462720
AN - SCOPUS:84893555489
VL - 109
SP - 160
EP - 168
JO - Neurobiology of Learning and Memory
JF - Neurobiology of Learning and Memory
SN - 1074-7427
ER -