Reduced activity-dependent protein levels in a mouse model of the fragile X premutation

Ramona E. von Leden, Lindsey C. Curley, Gian D. Greenberg, Michael R. Hunsaker, Rob Willemsen, Robert F Berman

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Environmental enrichment results in increased levels of Fmrp in brain and increased dendritic complexity. The present experiment evaluated activity-dependent increases in Fmrp levels in the motor cortex in response to training on a skilled forelimb reaching task in the CGG KI mouse model of the fragile X premutation. Fmrp, Arc, and c-Fos protein levels were quantified by Western blot in the contralateral motor cortex of mice following training to reach for sucrose pellets with a non-preferred paw and compared to levels in the ipsilateral motor cortex. After training, all mice showed increases in Fmrp, Arc, and c-Fos protein levels in the contralateral compared to the ipsilateral hemisphere; however, the increase in CGG KI mice was less than wildtype mice. Increases in Fmrp and Arc proteins scaled with learning, whereas this relationship was not observed with the c-Fos levels. These data suggest the possibility that reduced levels of activity-dependent proteins associated with synaptic plasticity such as Fmrp and Arc may contribute to the neurocognitive phenotype reported in the CGG KI mice and the fragile X premutation.

Original languageEnglish (US)
Pages (from-to)160-168
Number of pages9
JournalNeurobiology of Learning and Memory
Volume109
DOIs
StatePublished - Mar 2014

Keywords

  • Arc
  • C-Fos
  • CGG KI mouse
  • Endophenotype
  • Fmrp
  • Fragile X premutation
  • Western blot

ASJC Scopus subject areas

  • Behavioral Neuroscience
  • Cognitive Neuroscience
  • Experimental and Cognitive Psychology

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