RecQ helicase, in concert with RecA and SSB proteins, initiates and disrupts DNA recombination

Frank G. Harmon, Stephen C. Kowalczykowski

Research output: Contribution to journalArticle

226 Scopus citations

Abstract

RecQ helicase is important to homologous recombination and DNA repair in Escherichia coli. We demonstrate that RecQ helicase, in conjunction with RecA and SSB proteins, can initiate recombination events in vitro. In addition, RecQ protein is capable of unwinding a wide variety of DNA substrates, including joint molecules formed by RecA protein. These data are consistent with RecQ helicase assuming two roles in the cell; it can be (1) an initiator of homologous recombination, or (2) a disrupter of joint molecules formed by aberrant recombination. These findings also shed light on the function of the eukaryotic homologs of RecQ helicase, the Sgs1, Blm, and Wrn helicases.

Original languageEnglish (US)
Pages (from-to)1134-1144
Number of pages11
JournalGenes and Development
Volume12
Issue number8
StatePublished - Apr 15 1998

Keywords

  • DNA repair
  • Helicase
  • RecA
  • Recombination
  • RecQ

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Fingerprint Dive into the research topics of 'RecQ helicase, in concert with RecA and SSB proteins, initiates and disrupts DNA recombination'. Together they form a unique fingerprint.

  • Cite this