Recovery of microorganisms from synovial and pleural fluids of animals using hyperosmolar media

A. Matheson Buchanan, D. C. Davis, Niels C Pedersen, B. L. Beaman

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

L-phase (CWD) broth and plate media were used in parallel with conventional microbiological media during a 3-year period for culturing synovial and pleural fluids of animals. Two kinds of recoveries were obtained where parallel conventional methods were negative: (1) parent or normal bacteria, in very low numbers; and (2) Type B CWD variants in equally low numbers. Organisms in group 1 were: Streptococcus zooepidemicus from horses (2X); β-hemolytic streptococci, Lancefield Gp. G (2X); Staphylococcus aureus; Actinobacillus, and Actinomyces viscosus. Group 2 consisted of Bacteroides sp., Propionibacterium acnes, and three "Nocardia-like" sp. Catalase + Actinomyces was not recovered equally well on CWD plates as on conventional media with fluids obtained during ampicillin treatment. This occurred in spite of the fact that the CWD media was shown to support growth and reversion of laboratory induced L-phase variants of Nocardia caviae and N. asteroides, and had facilitated recovery of a Bacteroides L-phase variant from a pleural fluid. The nature of this fault in the media is under investigation in this laboratory.

Original languageEnglish (US)
Pages (from-to)19-33
Number of pages15
JournalVeterinary Microbiology
Volume7
Issue number1
DOIs
StatePublished - 1982

Fingerprint

Nocardia
Bacteroides
Synovial Fluid
Actinobacillus
Actinomyces viscosus
Streptococcus equi
microorganisms
Propionibacterium acnes
Actinomyces
Nocardia otitidiscaviarum
Ampicillin
Streptococcus
Nocardia asteroides
Catalase
Streptococcus equi subsp. zooepidemicus
Horses
Staphylococcus aureus
animals
ampicillin
Bacteria

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

Cite this

Recovery of microorganisms from synovial and pleural fluids of animals using hyperosmolar media. / Matheson Buchanan, A.; Davis, D. C.; Pedersen, Niels C; Beaman, B. L.

In: Veterinary Microbiology, Vol. 7, No. 1, 1982, p. 19-33.

Research output: Contribution to journalArticle

Matheson Buchanan, A. ; Davis, D. C. ; Pedersen, Niels C ; Beaman, B. L. / Recovery of microorganisms from synovial and pleural fluids of animals using hyperosmolar media. In: Veterinary Microbiology. 1982 ; Vol. 7, No. 1. pp. 19-33.
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