Recovery and cultivation of keratinocytes from shipped mouse skin

Hsin ya Yang, Thi Dinh La, Zhanna Gurenko, Pieter Steenhuis, Wei Liu, Roslyn Rivkah Isseroff

Research output: Contribution to journalArticlepeer-review

Abstract

Murine keratinocyte culture from neonatal skin is an important tool for studying the functional role of specific genes in epithelial biology. However, when the transgenic animal is only available in a geographically distant local, obtaining viable keratinocytes can be problematic. A method for transferring the isolated murine skin from collaborating labs could decrease the cost of shipping live animals, and would allow the efficient use of the tissues from the transgenic animals. Here we optimized shipping conditions and characterized the cells retrieved and cultured from mouse skin shipped for 48h at 0°C. The cultured keratinocytes from the control, non-shipped skin and the 2-day shipped skin were 43.6+/-7.8% viable, doubled every 2 days, and expressed comparable amounts of heat shock proteins and CD29/integrin beta-1. However, under the same shipping conditions, the 3-day shipped tissue failed to establish colonies in the culture. Therefore, this 2-day shipping technique allows the transfer mouse skin from distant locations with recovery of viable, propagatable keratinocytes, facilitating long-distance collaborations. J. Cell. Physiol. 230: 242-245, 2015.

Original languageEnglish (US)
Pages (from-to)242-245
Number of pages4
JournalJournal of Cellular Physiology
Volume230
Issue number2
DOIs
StatePublished - Feb 1 2015

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

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