Abstract
The Ca2+-binding protein recoverin may regulate visual transduction in retinal rods and cones, but its functional role and mechanism of action remain controversial. We compared the photoresponses of rods from control mice and from mice in which the recoverin gene was knocked out. Our analysis indicates that Ca2+-recoverin prolongs the dark-adapted flash response and increases the rod's sensitivity to dim steady light. Knockout rods had faster Ca2+ dynamics, indicating that recoverin is a significant Ca2+ buffer in the outer segment, but incorporation of exogenous buffer did not restore wild-type behavior. We infer that Ca 2+-recoverin potentiates light-triggered phosphodiesterase activity, probably by effectively prolonging the catalytic activity of photoexcited rhodopsin.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 729-741 |
| Number of pages | 13 |
| Journal | Journal of General Physiology |
| Volume | 123 |
| Issue number | 6 |
| DOIs | |
| State | Published - Jun 2004 |
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Keywords
- Calcium-binding proteins
- Knockout mice
- Light adaptation
- Photoreceptors
- Phototransduction
ASJC Scopus subject areas
- Physiology
Cite this
Recoverin regulates light-dependent phosphodiesterase activity in retinal rods. / Makino, Clint L.; Dodd, R. L.; Chen, J.; Burns, Marie E; Roca, A.; Simon, M. I.; Baylor, D. A.
In: Journal of General Physiology, Vol. 123, No. 6, 06.2004, p. 729-741.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Recoverin regulates light-dependent phosphodiesterase activity in retinal rods
AU - Makino, Clint L.
AU - Dodd, R. L.
AU - Chen, J.
AU - Burns, Marie E
AU - Roca, A.
AU - Simon, M. I.
AU - Baylor, D. A.
PY - 2004/6
Y1 - 2004/6
N2 - The Ca2+-binding protein recoverin may regulate visual transduction in retinal rods and cones, but its functional role and mechanism of action remain controversial. We compared the photoresponses of rods from control mice and from mice in which the recoverin gene was knocked out. Our analysis indicates that Ca2+-recoverin prolongs the dark-adapted flash response and increases the rod's sensitivity to dim steady light. Knockout rods had faster Ca2+ dynamics, indicating that recoverin is a significant Ca2+ buffer in the outer segment, but incorporation of exogenous buffer did not restore wild-type behavior. We infer that Ca 2+-recoverin potentiates light-triggered phosphodiesterase activity, probably by effectively prolonging the catalytic activity of photoexcited rhodopsin.
AB - The Ca2+-binding protein recoverin may regulate visual transduction in retinal rods and cones, but its functional role and mechanism of action remain controversial. We compared the photoresponses of rods from control mice and from mice in which the recoverin gene was knocked out. Our analysis indicates that Ca2+-recoverin prolongs the dark-adapted flash response and increases the rod's sensitivity to dim steady light. Knockout rods had faster Ca2+ dynamics, indicating that recoverin is a significant Ca2+ buffer in the outer segment, but incorporation of exogenous buffer did not restore wild-type behavior. We infer that Ca 2+-recoverin potentiates light-triggered phosphodiesterase activity, probably by effectively prolonging the catalytic activity of photoexcited rhodopsin.
KW - Calcium-binding proteins
KW - Knockout mice
KW - Light adaptation
KW - Photoreceptors
KW - Phototransduction
UR - http://www.scopus.com/inward/record.url?scp=2942659139&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=2942659139&partnerID=8YFLogxK
U2 - 10.1085/jgp.200308994
DO - 10.1085/jgp.200308994
M3 - Article
C2 - 15173221
AN - SCOPUS:2942659139
VL - 123
SP - 729
EP - 741
JO - Journal of General Physiology
JF - Journal of General Physiology
SN - 0022-1295
IS - 6
ER -