Recoverin regulates light-dependent phosphodiesterase activity in retinal rods

Clint L. Makino, R. L. Dodd, J. Chen, M. E. Burns, A. Roca, M. I. Simon, D. A. Baylor

Research output: Contribution to journalArticlepeer-review

132 Scopus citations


The Ca2+-binding protein recoverin may regulate visual transduction in retinal rods and cones, but its functional role and mechanism of action remain controversial. We compared the photoresponses of rods from control mice and from mice in which the recoverin gene was knocked out. Our analysis indicates that Ca2+-recoverin prolongs the dark-adapted flash response and increases the rod's sensitivity to dim steady light. Knockout rods had faster Ca2+ dynamics, indicating that recoverin is a significant Ca2+ buffer in the outer segment, but incorporation of exogenous buffer did not restore wild-type behavior. We infer that Ca 2+-recoverin potentiates light-triggered phosphodiesterase activity, probably by effectively prolonging the catalytic activity of photoexcited rhodopsin.

Original languageEnglish (US)
Pages (from-to)729-741
Number of pages13
JournalJournal of General Physiology
Issue number6
StatePublished - Jun 2004
Externally publishedYes


  • Calcium-binding proteins
  • Knockout mice
  • Light adaptation
  • Photoreceptors
  • Phototransduction

ASJC Scopus subject areas

  • Physiology


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