TY - JOUR
T1 - Reconstitution of an SOS response pathway
T2 - Derepression of transcription in response to DNA breaks
AU - Anderson, Daniel G.
AU - Kowalczykowski, Stephen C.
PY - 1998/12/23
Y1 - 1998/12/23
N2 - E. coli responds to DNA damage by derepressing the transcription of about 20 genes that make up the SOS pathway. Genetic analyses have shown that SOS induction in response to double-stranded DNA (dsDNA) breaks requires LexA repressor, and the RecA and RecBCD enzymes-proteins best known for their role as initiators of dsDNA break repair and homologous recombination. Here we demonstrate that purified RecA protein, RecBCD enzyme, single-stranded DNA- binding (SSB) protein, and LexA repressor respond to dsDNA breaks in vitro by derepressing transcription from an SOS promoter. Interestingly, derepression is more rapid if the DNA containing the dsDNA break has a X recombination hot spot (5'-GCTGGTGG,3'), suggesting a novel regulatory role for one of the most overrepresented octamers in the E. coli genome.
AB - E. coli responds to DNA damage by derepressing the transcription of about 20 genes that make up the SOS pathway. Genetic analyses have shown that SOS induction in response to double-stranded DNA (dsDNA) breaks requires LexA repressor, and the RecA and RecBCD enzymes-proteins best known for their role as initiators of dsDNA break repair and homologous recombination. Here we demonstrate that purified RecA protein, RecBCD enzyme, single-stranded DNA- binding (SSB) protein, and LexA repressor respond to dsDNA breaks in vitro by derepressing transcription from an SOS promoter. Interestingly, derepression is more rapid if the DNA containing the dsDNA break has a X recombination hot spot (5'-GCTGGTGG,3'), suggesting a novel regulatory role for one of the most overrepresented octamers in the E. coli genome.
UR - http://www.scopus.com/inward/record.url?scp=0032428175&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032428175&partnerID=8YFLogxK
U2 - 10.1016/S0092-8674(00)81721-3
DO - 10.1016/S0092-8674(00)81721-3
M3 - Article
C2 - 9875851
AN - SCOPUS:0032428175
VL - 95
SP - 975
EP - 979
JO - Cell
JF - Cell
SN - 0092-8674
IS - 7
ER -