Reconstituting ring-rafts in bud-mimicking topography of model membranes

Yong Sang Ryu, In Ho Lee, Jeng Hun Suh, Seung Chul Park, Soojung Oh, Luke R. Jordan, Nathan J. Wittenberg, Sang Hyun Oh, Noo Li Jeon, Byoungho Lee, Atul N. Parikh, Sin Doo Lee

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

During vesicular trafficking and release of enveloped viruses, the budding and fission processes dynamically remodel the donor cell membrane in a protein-or a lipid-mediated manner. In all cases, in addition to the generation or relief of the curvature stress, the buds recruit specific lipids and proteins from the donor membrane through restricted diffusion for the development of a ring-type raft domain of closed topology. Here, by reconstituting the bud topography in a model membrane, we demonstrate the preferential localization of cholesterol-and sphingomyelin-enriched microdomains in the collar band of the bud-neck interfaced with the donor membrane. The geometrical approach to the recapitulation of the dynamic membrane reorganization, resulting from the local radii of curvatures from nanometre-to-micrometre scales, offers important clues for understanding the active roles of the bud topography in the sorting and migration machinery of key signalling proteins involved in membrane budding.

Original languageEnglish (US)
Article number4507
JournalNature Communications
Volume5
DOIs
StatePublished - Jul 24 2014

Fingerprint

rafts
Topography
topography
membranes
Membranes
rings
proteins
lipids
Virus Release
Lipids
curvature
Sphingomyelins
Proteins
cholesterol
machinery
viruses
Cell membranes
Membrane Proteins
classifying
Neck

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Physics and Astronomy(all)

Cite this

Ryu, Y. S., Lee, I. H., Suh, J. H., Park, S. C., Oh, S., Jordan, L. R., ... Lee, S. D. (2014). Reconstituting ring-rafts in bud-mimicking topography of model membranes. Nature Communications, 5, [4507]. https://doi.org/10.1038/ncomms5507

Reconstituting ring-rafts in bud-mimicking topography of model membranes. / Ryu, Yong Sang; Lee, In Ho; Suh, Jeng Hun; Park, Seung Chul; Oh, Soojung; Jordan, Luke R.; Wittenberg, Nathan J.; Oh, Sang Hyun; Jeon, Noo Li; Lee, Byoungho; Parikh, Atul N.; Lee, Sin Doo.

In: Nature Communications, Vol. 5, 4507, 24.07.2014.

Research output: Contribution to journalArticle

Ryu, YS, Lee, IH, Suh, JH, Park, SC, Oh, S, Jordan, LR, Wittenberg, NJ, Oh, SH, Jeon, NL, Lee, B, Parikh, AN & Lee, SD 2014, 'Reconstituting ring-rafts in bud-mimicking topography of model membranes', Nature Communications, vol. 5, 4507. https://doi.org/10.1038/ncomms5507
Ryu, Yong Sang ; Lee, In Ho ; Suh, Jeng Hun ; Park, Seung Chul ; Oh, Soojung ; Jordan, Luke R. ; Wittenberg, Nathan J. ; Oh, Sang Hyun ; Jeon, Noo Li ; Lee, Byoungho ; Parikh, Atul N. ; Lee, Sin Doo. / Reconstituting ring-rafts in bud-mimicking topography of model membranes. In: Nature Communications. 2014 ; Vol. 5.
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