Reconfigurable microfluidic dilution for high-throughput quantitative assays

Jinzhen Fan, Baoqing Li, Siyuan Xing, Tingrui Pan

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

This paper reports a reconfigurable microfluidic dilution device for high-throughput quantitative assays, which can easily produce discrete logarithmic/binary concentration profiles ranging from 1 to 100-fold dilution in parallel from a fixed sample volume (e.g., 10 μL) without any assistance of continuous fluidic pump or robotic automation. The integrated dilution generation chip consists of switchable distribution and collection channels, metering reservoirs, reaction chambers, and pressure-activatable Laplace valves. Following the sequential loading of a sample, a diluent, and a detection reagent into their individual metering chambers, the top microfluidic layer can be reconfigured to collect the metered chemicals into the reaction chambers in parallel, where detection will be conducted. To facilitate mixing and reaction in the microchambers, two acoustic microstreaming actuation mechanisms have been investigated for easy integrability and accessibility. Furthermore, the microfluidic dilution generator has been characterized by both colorimetric and fluorescent means. A further demonstration of the generic usage of the quantitative dilution chip has utilized the commonly available bicinchoninic acid (BCA) assay to analyse the protein concentrations of human tissue extracts. In brief, the microfluidic dilution generator offers a high-throughput high-efficiency quantitative analytical alternative to conventional quantitative assay platforms, by simple manipulation of a minute amount of chemicals in a compact microfluidic device with minimal equipment requirement, which can serve as a facile tool for biochemical and biological analyses in regular laboratories, point-of-care settings and low-resource environments.

Original languageEnglish (US)
Pages (from-to)2670-2679
Number of pages10
JournalLab on a Chip
Volume15
Issue number12
DOIs
StatePublished - Jun 21 2015

Fingerprint

Lab-On-A-Chip Devices
Microfluidics
Dilution
Assays
Throughput
Point-of-Care Systems
Tissue Extracts
Automation
Robotics
Acoustics
Pressure
Equipment and Supplies
Fluidics
Proteins
Demonstrations
Pumps
Tissue
Acids

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Cite this

Reconfigurable microfluidic dilution for high-throughput quantitative assays. / Fan, Jinzhen; Li, Baoqing; Xing, Siyuan; Pan, Tingrui.

In: Lab on a Chip, Vol. 15, No. 12, 21.06.2015, p. 2670-2679.

Research output: Contribution to journalArticle

Fan, Jinzhen ; Li, Baoqing ; Xing, Siyuan ; Pan, Tingrui. / Reconfigurable microfluidic dilution for high-throughput quantitative assays. In: Lab on a Chip. 2015 ; Vol. 15, No. 12. pp. 2670-2679.
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