Recombination following superinfection by HIV-1

Guowei Fang, Barbara Weiser, Carla Kuiken, Sean M. Philpott, Sarah Rowland-Jones, Francis Plummer, Joshua Kimani, Binshan Shi, Rupert Kaul, Job Bwayo, Omu Anzala, Harold Burger

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

Background: There is increasing recognition of recombinant HIV-1 strains globally, but it has been unclear whether recombination results from superinfection during untreated, chronic infection. Objective: To search for evidence of recombination and superinfection in Africa, where multiple HIV-1 subtypes facilitate identification of strains. Methods: Serial blood samples from highly exposed, chronically infected women in Nairobi's Pumwani sex workers cohort were examined. Serial, complete HIV-1 RNA sequence analyses were performed for seven untreated long-term survivors. Sequences were subjected to computational analysis. Results: One woman had evidence of both superinfection and recombination. Complete HIV-1 RNA sequences were first derived from plasma obtained in 1986, when the woman had been HIV seropositive for at least 21 months; this sequence was entirely subtype A. The sequences obtained from plasma in 1995 and 1997, however, were subtype A/C recombinants with a SimPlot demonstrating that the subtype A fragment in 1995 and 1997 was derived from the original 1986 A sequence. Heteroduplex tracking assays demonstrated that the subtype C sequences were not detectable as minor species in 1986. Conclusion: Intersubtype recombination took place between the original non-recombinant subtype A strain and the superinfecting subtype C strain in an untreated, chronically infected woman. This finding helps to explain the rising prevalence of recombinant HIV-1 worldwide. Recombination resulting from superinfection with diverse strains may pose problems for eliciting broad immune responses necessary for an effective vaccine.

Original languageEnglish (US)
Pages (from-to)153-159
Number of pages7
JournalAIDS
Volume18
Issue number2
DOIs
StatePublished - Jan 23 2004
Externally publishedYes

Fingerprint

Superinfection
Genetic Recombination
HIV-1
RNA Sequence Analysis
Sex Workers
Survivors
Vaccines
HIV
Infection

Keywords

  • Africa
  • HIV-1
  • Recombination
  • Superinfection
  • Vaccine
  • Women

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Fang, G., Weiser, B., Kuiken, C., Philpott, S. M., Rowland-Jones, S., Plummer, F., ... Burger, H. (2004). Recombination following superinfection by HIV-1. AIDS, 18(2), 153-159. https://doi.org/10.1097/00002030-200401230-00003

Recombination following superinfection by HIV-1. / Fang, Guowei; Weiser, Barbara; Kuiken, Carla; Philpott, Sean M.; Rowland-Jones, Sarah; Plummer, Francis; Kimani, Joshua; Shi, Binshan; Kaul, Rupert; Bwayo, Job; Anzala, Omu; Burger, Harold.

In: AIDS, Vol. 18, No. 2, 23.01.2004, p. 153-159.

Research output: Contribution to journalArticle

Fang, G, Weiser, B, Kuiken, C, Philpott, SM, Rowland-Jones, S, Plummer, F, Kimani, J, Shi, B, Kaul, R, Bwayo, J, Anzala, O & Burger, H 2004, 'Recombination following superinfection by HIV-1', AIDS, vol. 18, no. 2, pp. 153-159. https://doi.org/10.1097/00002030-200401230-00003
Fang G, Weiser B, Kuiken C, Philpott SM, Rowland-Jones S, Plummer F et al. Recombination following superinfection by HIV-1. AIDS. 2004 Jan 23;18(2):153-159. https://doi.org/10.1097/00002030-200401230-00003
Fang, Guowei ; Weiser, Barbara ; Kuiken, Carla ; Philpott, Sean M. ; Rowland-Jones, Sarah ; Plummer, Francis ; Kimani, Joshua ; Shi, Binshan ; Kaul, Rupert ; Bwayo, Job ; Anzala, Omu ; Burger, Harold. / Recombination following superinfection by HIV-1. In: AIDS. 2004 ; Vol. 18, No. 2. pp. 153-159.
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