Recombination following superinfection by HIV-1

Guowei Fang, Barbara Weiser, Carla Kuiken, Sean M. Philpott, Sarah Rowland-Jones, Francis Plummer, Joshua Kimani, Binshan Shi, Rupert Kaul, Job Bwayo, Omu Anzala, Harold Burger

Research output: Contribution to journalArticle

104 Scopus citations

Abstract

Background: There is increasing recognition of recombinant HIV-1 strains globally, but it has been unclear whether recombination results from superinfection during untreated, chronic infection. Objective: To search for evidence of recombination and superinfection in Africa, where multiple HIV-1 subtypes facilitate identification of strains. Methods: Serial blood samples from highly exposed, chronically infected women in Nairobi's Pumwani sex workers cohort were examined. Serial, complete HIV-1 RNA sequence analyses were performed for seven untreated long-term survivors. Sequences were subjected to computational analysis. Results: One woman had evidence of both superinfection and recombination. Complete HIV-1 RNA sequences were first derived from plasma obtained in 1986, when the woman had been HIV seropositive for at least 21 months; this sequence was entirely subtype A. The sequences obtained from plasma in 1995 and 1997, however, were subtype A/C recombinants with a SimPlot demonstrating that the subtype A fragment in 1995 and 1997 was derived from the original 1986 A sequence. Heteroduplex tracking assays demonstrated that the subtype C sequences were not detectable as minor species in 1986. Conclusion: Intersubtype recombination took place between the original non-recombinant subtype A strain and the superinfecting subtype C strain in an untreated, chronically infected woman. This finding helps to explain the rising prevalence of recombinant HIV-1 worldwide. Recombination resulting from superinfection with diverse strains may pose problems for eliciting broad immune responses necessary for an effective vaccine.

Original languageEnglish (US)
Pages (from-to)153-159
Number of pages7
JournalAIDS
Volume18
Issue number2
DOIs
StatePublished - Jan 23 2004
Externally publishedYes

Keywords

  • Africa
  • HIV-1
  • Recombination
  • Superinfection
  • Vaccine
  • Women

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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  • Cite this

    Fang, G., Weiser, B., Kuiken, C., Philpott, S. M., Rowland-Jones, S., Plummer, F., Kimani, J., Shi, B., Kaul, R., Bwayo, J., Anzala, O., & Burger, H. (2004). Recombination following superinfection by HIV-1. AIDS, 18(2), 153-159. https://doi.org/10.1097/00002030-200401230-00003