Recombinant polypeptide from the endonuclease region of the acquired immune deficiency syndrome retrovirus polymerase (pol) gene detects serum antibodies in most infected individuals

K. S. Steimer, K. W. Higgins, M. A. Powers, J. C. Stephans, A. Gyenes, C. George-Nascimento, Paul A Luciw, P. J. Barr, R. A. Hallewell, R. Sanchez-Pescador

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Sera from the majority of individuals that were positive in an enzyme-linked immunosorbent assay (ELISA) for antibodies to acquired immune deficiency syndrome (AIDS)-associated retrovirus (ARV), an isolate of the retrovirus identified as the etiologic agent of AIDS, were found to react with a 31,000-dalton protein (p31) in virus Western blot assays. To determine if this 31,000-dalton immunoreactive species originated from the putative endonuclease region of the polymerase (pol) gene of ARV, we cloned this portion of pol into bacterial expression vectors for direct expression and for expression as a fusion protein with human superoxide dismutase. Transformants from both constructions expressed immunoreactive protein detected in immunoblots with an AIDS patient's serum. Extracts from transformants expressing these sequences competed with the binding of antibodies from AIDS patients' sera to the 31,000-dalton protein in virus immunoblots, confirming that viral p31 originated from the endonuclease domain of the ARV polymerase gene. The superoxide dismutase-p31 fusion protein was purified, and an ELISA for detecting antibodies to p31 was developed. The majority (95%) of serum samples obtained from individuals seropositive in the virus ELISA were also positive in the p31 antibody ELISA.

Original languageEnglish (US)
Pages (from-to)9-16
Number of pages8
JournalJournal of Virology
Volume58
Issue number1
StatePublished - 1986

Fingerprint

Retroviridae
acquired immunodeficiency syndrome
Endonucleases
polypeptides
Acquired Immunodeficiency Syndrome
Peptides
antibodies
Enzyme-Linked Immunosorbent Assay
Antibodies
enzyme-linked immunosorbent assay
Serum
Genes
Viruses
Proteins
genes
proteins
viruses
Superoxide Dismutase
superoxide dismutase
Western blotting

ASJC Scopus subject areas

  • Immunology

Cite this

Steimer, K. S., Higgins, K. W., Powers, M. A., Stephans, J. C., Gyenes, A., George-Nascimento, C., ... Sanchez-Pescador, R. (1986). Recombinant polypeptide from the endonuclease region of the acquired immune deficiency syndrome retrovirus polymerase (pol) gene detects serum antibodies in most infected individuals. Journal of Virology, 58(1), 9-16.

Recombinant polypeptide from the endonuclease region of the acquired immune deficiency syndrome retrovirus polymerase (pol) gene detects serum antibodies in most infected individuals. / Steimer, K. S.; Higgins, K. W.; Powers, M. A.; Stephans, J. C.; Gyenes, A.; George-Nascimento, C.; Luciw, Paul A; Barr, P. J.; Hallewell, R. A.; Sanchez-Pescador, R.

In: Journal of Virology, Vol. 58, No. 1, 1986, p. 9-16.

Research output: Contribution to journalArticle

Steimer, KS, Higgins, KW, Powers, MA, Stephans, JC, Gyenes, A, George-Nascimento, C, Luciw, PA, Barr, PJ, Hallewell, RA & Sanchez-Pescador, R 1986, 'Recombinant polypeptide from the endonuclease region of the acquired immune deficiency syndrome retrovirus polymerase (pol) gene detects serum antibodies in most infected individuals', Journal of Virology, vol. 58, no. 1, pp. 9-16.
Steimer, K. S. ; Higgins, K. W. ; Powers, M. A. ; Stephans, J. C. ; Gyenes, A. ; George-Nascimento, C. ; Luciw, Paul A ; Barr, P. J. ; Hallewell, R. A. ; Sanchez-Pescador, R. / Recombinant polypeptide from the endonuclease region of the acquired immune deficiency syndrome retrovirus polymerase (pol) gene detects serum antibodies in most infected individuals. In: Journal of Virology. 1986 ; Vol. 58, No. 1. pp. 9-16.
@article{c3567bd031af469fb6c485221c5b0577,
title = "Recombinant polypeptide from the endonuclease region of the acquired immune deficiency syndrome retrovirus polymerase (pol) gene detects serum antibodies in most infected individuals",
abstract = "Sera from the majority of individuals that were positive in an enzyme-linked immunosorbent assay (ELISA) for antibodies to acquired immune deficiency syndrome (AIDS)-associated retrovirus (ARV), an isolate of the retrovirus identified as the etiologic agent of AIDS, were found to react with a 31,000-dalton protein (p31) in virus Western blot assays. To determine if this 31,000-dalton immunoreactive species originated from the putative endonuclease region of the polymerase (pol) gene of ARV, we cloned this portion of pol into bacterial expression vectors for direct expression and for expression as a fusion protein with human superoxide dismutase. Transformants from both constructions expressed immunoreactive protein detected in immunoblots with an AIDS patient's serum. Extracts from transformants expressing these sequences competed with the binding of antibodies from AIDS patients' sera to the 31,000-dalton protein in virus immunoblots, confirming that viral p31 originated from the endonuclease domain of the ARV polymerase gene. The superoxide dismutase-p31 fusion protein was purified, and an ELISA for detecting antibodies to p31 was developed. The majority (95{\%}) of serum samples obtained from individuals seropositive in the virus ELISA were also positive in the p31 antibody ELISA.",
author = "Steimer, {K. S.} and Higgins, {K. W.} and Powers, {M. A.} and Stephans, {J. C.} and A. Gyenes and C. George-Nascimento and Luciw, {Paul A} and Barr, {P. J.} and Hallewell, {R. A.} and R. Sanchez-Pescador",
year = "1986",
language = "English (US)",
volume = "58",
pages = "9--16",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "1",

}

TY - JOUR

T1 - Recombinant polypeptide from the endonuclease region of the acquired immune deficiency syndrome retrovirus polymerase (pol) gene detects serum antibodies in most infected individuals

AU - Steimer, K. S.

AU - Higgins, K. W.

AU - Powers, M. A.

AU - Stephans, J. C.

AU - Gyenes, A.

AU - George-Nascimento, C.

AU - Luciw, Paul A

AU - Barr, P. J.

AU - Hallewell, R. A.

AU - Sanchez-Pescador, R.

PY - 1986

Y1 - 1986

N2 - Sera from the majority of individuals that were positive in an enzyme-linked immunosorbent assay (ELISA) for antibodies to acquired immune deficiency syndrome (AIDS)-associated retrovirus (ARV), an isolate of the retrovirus identified as the etiologic agent of AIDS, were found to react with a 31,000-dalton protein (p31) in virus Western blot assays. To determine if this 31,000-dalton immunoreactive species originated from the putative endonuclease region of the polymerase (pol) gene of ARV, we cloned this portion of pol into bacterial expression vectors for direct expression and for expression as a fusion protein with human superoxide dismutase. Transformants from both constructions expressed immunoreactive protein detected in immunoblots with an AIDS patient's serum. Extracts from transformants expressing these sequences competed with the binding of antibodies from AIDS patients' sera to the 31,000-dalton protein in virus immunoblots, confirming that viral p31 originated from the endonuclease domain of the ARV polymerase gene. The superoxide dismutase-p31 fusion protein was purified, and an ELISA for detecting antibodies to p31 was developed. The majority (95%) of serum samples obtained from individuals seropositive in the virus ELISA were also positive in the p31 antibody ELISA.

AB - Sera from the majority of individuals that were positive in an enzyme-linked immunosorbent assay (ELISA) for antibodies to acquired immune deficiency syndrome (AIDS)-associated retrovirus (ARV), an isolate of the retrovirus identified as the etiologic agent of AIDS, were found to react with a 31,000-dalton protein (p31) in virus Western blot assays. To determine if this 31,000-dalton immunoreactive species originated from the putative endonuclease region of the polymerase (pol) gene of ARV, we cloned this portion of pol into bacterial expression vectors for direct expression and for expression as a fusion protein with human superoxide dismutase. Transformants from both constructions expressed immunoreactive protein detected in immunoblots with an AIDS patient's serum. Extracts from transformants expressing these sequences competed with the binding of antibodies from AIDS patients' sera to the 31,000-dalton protein in virus immunoblots, confirming that viral p31 originated from the endonuclease domain of the ARV polymerase gene. The superoxide dismutase-p31 fusion protein was purified, and an ELISA for detecting antibodies to p31 was developed. The majority (95%) of serum samples obtained from individuals seropositive in the virus ELISA were also positive in the p31 antibody ELISA.

UR - http://www.scopus.com/inward/record.url?scp=0022530486&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022530486&partnerID=8YFLogxK

M3 - Article

C2 - 2419593

AN - SCOPUS:0022530486

VL - 58

SP - 9

EP - 16

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 1

ER -