Recombinant juvenile hormone esterase as a biochemical anti-juvenile hormone agent: Effects on ovarian development in Acheta domesticus

Bryony C. Bonning, Werner Loher, Bruce D. Hammock

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

By investigating the effects of recombinant juvenile hormone esterase (JHE) on the stimulation of ovarian development and egg laying in the house cricket Acheta domesticus L, we have tested the hypothesis that recombinant JHE (derived from the tobacco budworm Heliothis virescens) can be used as a biochemical anti-juvenile hormone (JH) agent. Recombinant JHE, produced by a genetically engineered baculovirus, was affinity-purified and injected into females of A. domesticus. JHE was cleared rapidly from the hemolymph of the crickets. However, upon repeated injection, significant reductions were seen in the extent of development of the ovaries and in the numbers of eggs laid. The effects of JHE could be rescued by topical application of the JHE inhibitor, OTFP. Thus, we have demonstrated an anti-JH effect on reproduction and that the recombinant JHE derived from a lepidopteran is active in an orthopteran insect.

Original languageEnglish (US)
Pages (from-to)359-368
Number of pages10
JournalArchives of Insect Biochemistry and Physiology
Volume34
Issue number3
StatePublished - 1997

Fingerprint

juvenile hormone esterase
Acheta domesticus
Juvenile Hormones
ovarian development
juvenile hormones
Gryllidae
Heliothis virescens
Tobacco
Hemolymph
Ovulation Induction
Baculoviridae
topical application
Eggs
hemolymph
Reproduction
Ovum
Insects
Ovary
oviposition
Lepidoptera

Keywords

  • Acheta domesticus
  • JHE pharmacokinetics
  • Recombinant JHE
  • Vitellogenesis

ASJC Scopus subject areas

  • Insect Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physiology
  • Physiology (medical)

Cite this

Recombinant juvenile hormone esterase as a biochemical anti-juvenile hormone agent : Effects on ovarian development in Acheta domesticus. / Bonning, Bryony C.; Loher, Werner; Hammock, Bruce D.

In: Archives of Insect Biochemistry and Physiology, Vol. 34, No. 3, 1997, p. 359-368.

Research output: Contribution to journalArticle

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