Recombinant juvenile hormone esterase as a biochemical anti-juvenile hormone agent: Effects on ovarian development in Acheta domesticus

Bryony C. Bonning, Werner Loher, Bruce D. Hammock

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

By investigating the effects of recombinant juvenile hormone esterase (JHE) on the stimulation of ovarian development and egg laying in the house cricket Acheta domesticus L, we have tested the hypothesis that recombinant JHE (derived from the tobacco budworm Heliothis virescens) can be used as a biochemical anti-juvenile hormone (JH) agent. Recombinant JHE, produced by a genetically engineered baculovirus, was affinity-purified and injected into females of A. domesticus. JHE was cleared rapidly from the hemolymph of the crickets. However, upon repeated injection, significant reductions were seen in the extent of development of the ovaries and in the numbers of eggs laid. The effects of JHE could be rescued by topical application of the JHE inhibitor, OTFP. Thus, we have demonstrated an anti-JH effect on reproduction and that the recombinant JHE derived from a lepidopteran is active in an orthopteran insect.

Original languageEnglish (US)
Pages (from-to)359-368
Number of pages10
JournalArchives of Insect Biochemistry and Physiology
Volume34
Issue number3
StatePublished - 1997

Keywords

  • Acheta domesticus
  • JHE pharmacokinetics
  • Recombinant JHE
  • Vitellogenesis

ASJC Scopus subject areas

  • Insect Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physiology
  • Physiology (medical)

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