Abstract
Recombinant reassortment technology was used to prepare H5N1 influenza vaccine strains containing a modified hemagglutinin (HA) gene and neuraminidase gene from the A/Hong Kong/156/97 and A/Hong Kong/483/97 isolates and the internal genes from the attenuated cold-adapted A/Ann Arbor/6/60 influenza virus strain. The HA cleavage site (HA1/HA2) of each HSN1 isolate was modified to resemble that of 'low-pathogenic' avian strains. Five of 6 basic amino acids at the cleavage site were deleted, and a threonine was added upstream of the remaining arginine. The H5 HA cleavage site modification resulted in the expected trypsin-dependent phenotype without altering the antigenic character of the H5 HA molecule. The temperature-sensitive and cold-adapted phenotype of the attenuated parent virus was maintained in the recombinant strains, and they grew to 108.5+9.4 EID50/mL in eggs. Both H5N1 vaccine virus strains were safe and immunogenic in ferrets and protected chickens against wild-type H5N1 virus challenge.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1132-1138 |
| Number of pages | 7 |
| Journal | Journal of Infectious Diseases |
| Volume | 179 |
| Issue number | 5 |
| DOIs | |
| State | Published - 1999 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Public Health, Environmental and Occupational Health
- Immunology
Cite this
Recombinant influenza A virus vaccines for the pathogenic human A/Hong Kong/97 (H5N1) viruses. / Shengqiang, Li; Chongguang, Liu; Klimov, Alexander; Subbarao, Kanta; Perdue, Michael L.; Delia, Mo; Yaying, Ji; Woods, Leslie; Hietala, Sharon; Bryant, Martin.
In: Journal of Infectious Diseases, Vol. 179, No. 5, 1999, p. 1132-1138.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Recombinant influenza A virus vaccines for the pathogenic human A/Hong Kong/97 (H5N1) viruses
AU - Shengqiang, Li
AU - Chongguang, Liu
AU - Klimov, Alexander
AU - Subbarao, Kanta
AU - Perdue, Michael L.
AU - Delia, Mo
AU - Yaying, Ji
AU - Woods, Leslie
AU - Hietala, Sharon
AU - Bryant, Martin
PY - 1999
Y1 - 1999
N2 - Recombinant reassortment technology was used to prepare H5N1 influenza vaccine strains containing a modified hemagglutinin (HA) gene and neuraminidase gene from the A/Hong Kong/156/97 and A/Hong Kong/483/97 isolates and the internal genes from the attenuated cold-adapted A/Ann Arbor/6/60 influenza virus strain. The HA cleavage site (HA1/HA2) of each HSN1 isolate was modified to resemble that of 'low-pathogenic' avian strains. Five of 6 basic amino acids at the cleavage site were deleted, and a threonine was added upstream of the remaining arginine. The H5 HA cleavage site modification resulted in the expected trypsin-dependent phenotype without altering the antigenic character of the H5 HA molecule. The temperature-sensitive and cold-adapted phenotype of the attenuated parent virus was maintained in the recombinant strains, and they grew to 108.5+9.4 EID50/mL in eggs. Both H5N1 vaccine virus strains were safe and immunogenic in ferrets and protected chickens against wild-type H5N1 virus challenge.
AB - Recombinant reassortment technology was used to prepare H5N1 influenza vaccine strains containing a modified hemagglutinin (HA) gene and neuraminidase gene from the A/Hong Kong/156/97 and A/Hong Kong/483/97 isolates and the internal genes from the attenuated cold-adapted A/Ann Arbor/6/60 influenza virus strain. The HA cleavage site (HA1/HA2) of each HSN1 isolate was modified to resemble that of 'low-pathogenic' avian strains. Five of 6 basic amino acids at the cleavage site were deleted, and a threonine was added upstream of the remaining arginine. The H5 HA cleavage site modification resulted in the expected trypsin-dependent phenotype without altering the antigenic character of the H5 HA molecule. The temperature-sensitive and cold-adapted phenotype of the attenuated parent virus was maintained in the recombinant strains, and they grew to 108.5+9.4 EID50/mL in eggs. Both H5N1 vaccine virus strains were safe and immunogenic in ferrets and protected chickens against wild-type H5N1 virus challenge.
UR - http://www.scopus.com/inward/record.url?scp=0033006537&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033006537&partnerID=8YFLogxK
U2 - 10.1086/314713
DO - 10.1086/314713
M3 - Article
C2 - 10191214
AN - SCOPUS:0033006537
VL - 179
SP - 1132
EP - 1138
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
SN - 0022-1899
IS - 5
ER -