Recombinant influenza A virus vaccines for the pathogenic human A/Hong Kong/97 (H5N1) viruses

Li Shengqiang, Liu Chongguang, Alexander Klimov, Kanta Subbarao, Michael L. Perdue, Mo Delia, Ji Yaying, Leslie Woods, Sharon Hietala, Martin Bryant

Research output: Contribution to journalArticle

107 Scopus citations

Abstract

Recombinant reassortment technology was used to prepare H5N1 influenza vaccine strains containing a modified hemagglutinin (HA) gene and neuraminidase gene from the A/Hong Kong/156/97 and A/Hong Kong/483/97 isolates and the internal genes from the attenuated cold-adapted A/Ann Arbor/6/60 influenza virus strain. The HA cleavage site (HA1/HA2) of each HSN1 isolate was modified to resemble that of 'low-pathogenic' avian strains. Five of 6 basic amino acids at the cleavage site were deleted, and a threonine was added upstream of the remaining arginine. The H5 HA cleavage site modification resulted in the expected trypsin-dependent phenotype without altering the antigenic character of the H5 HA molecule. The temperature-sensitive and cold-adapted phenotype of the attenuated parent virus was maintained in the recombinant strains, and they grew to 108.5+9.4 EID50/mL in eggs. Both H5N1 vaccine virus strains were safe and immunogenic in ferrets and protected chickens against wild-type H5N1 virus challenge.

Original languageEnglish (US)
Pages (from-to)1132-1138
Number of pages7
JournalJournal of Infectious Diseases
Volume179
Issue number5
DOIs
StatePublished - 1999
Externally publishedYes

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

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    Shengqiang, L., Chongguang, L., Klimov, A., Subbarao, K., Perdue, M. L., Delia, M., Yaying, J., Woods, L., Hietala, S., & Bryant, M. (1999). Recombinant influenza A virus vaccines for the pathogenic human A/Hong Kong/97 (H5N1) viruses. Journal of Infectious Diseases, 179(5), 1132-1138. https://doi.org/10.1086/314713