Recombinant influenza A virus vaccines for the pathogenic human A/Hong Kong/97 (H5N1) viruses

Li Shengqiang, Liu Chongguang, Alexander Klimov, Kanta Subbarao, Michael L. Perdue, Mo Delia, Ji Yaying, Leslie Woods, Sharon Hietala, Martin Bryant

Research output: Contribution to journalArticle

106 Citations (Scopus)

Abstract

Recombinant reassortment technology was used to prepare H5N1 influenza vaccine strains containing a modified hemagglutinin (HA) gene and neuraminidase gene from the A/Hong Kong/156/97 and A/Hong Kong/483/97 isolates and the internal genes from the attenuated cold-adapted A/Ann Arbor/6/60 influenza virus strain. The HA cleavage site (HA1/HA2) of each HSN1 isolate was modified to resemble that of 'low-pathogenic' avian strains. Five of 6 basic amino acids at the cleavage site were deleted, and a threonine was added upstream of the remaining arginine. The H5 HA cleavage site modification resulted in the expected trypsin-dependent phenotype without altering the antigenic character of the H5 HA molecule. The temperature-sensitive and cold-adapted phenotype of the attenuated parent virus was maintained in the recombinant strains, and they grew to 108.5+9.4 EID50/mL in eggs. Both H5N1 vaccine virus strains were safe and immunogenic in ferrets and protected chickens against wild-type H5N1 virus challenge.

Original languageEnglish (US)
Pages (from-to)1132-1138
Number of pages7
JournalJournal of Infectious Diseases
Volume179
Issue number5
DOIs
StatePublished - 1999
Externally publishedYes

Fingerprint

H5N1 Subtype Influenza A Virus
Influenza Vaccines
Influenza A virus
Hong Kong
Hemagglutinins
Genes
Phenotype
Basic Amino Acids
Ferrets
Neuraminidase
Threonine
Orthomyxoviridae
Trypsin
Eggs
Arginine
Chickens
Vaccines
Viruses
Technology
avian influenza A virus hemagglutinin

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Shengqiang, L., Chongguang, L., Klimov, A., Subbarao, K., Perdue, M. L., Delia, M., ... Bryant, M. (1999). Recombinant influenza A virus vaccines for the pathogenic human A/Hong Kong/97 (H5N1) viruses. Journal of Infectious Diseases, 179(5), 1132-1138. https://doi.org/10.1086/314713

Recombinant influenza A virus vaccines for the pathogenic human A/Hong Kong/97 (H5N1) viruses. / Shengqiang, Li; Chongguang, Liu; Klimov, Alexander; Subbarao, Kanta; Perdue, Michael L.; Delia, Mo; Yaying, Ji; Woods, Leslie; Hietala, Sharon; Bryant, Martin.

In: Journal of Infectious Diseases, Vol. 179, No. 5, 1999, p. 1132-1138.

Research output: Contribution to journalArticle

Shengqiang, L, Chongguang, L, Klimov, A, Subbarao, K, Perdue, ML, Delia, M, Yaying, J, Woods, L, Hietala, S & Bryant, M 1999, 'Recombinant influenza A virus vaccines for the pathogenic human A/Hong Kong/97 (H5N1) viruses', Journal of Infectious Diseases, vol. 179, no. 5, pp. 1132-1138. https://doi.org/10.1086/314713
Shengqiang L, Chongguang L, Klimov A, Subbarao K, Perdue ML, Delia M et al. Recombinant influenza A virus vaccines for the pathogenic human A/Hong Kong/97 (H5N1) viruses. Journal of Infectious Diseases. 1999;179(5):1132-1138. https://doi.org/10.1086/314713
Shengqiang, Li ; Chongguang, Liu ; Klimov, Alexander ; Subbarao, Kanta ; Perdue, Michael L. ; Delia, Mo ; Yaying, Ji ; Woods, Leslie ; Hietala, Sharon ; Bryant, Martin. / Recombinant influenza A virus vaccines for the pathogenic human A/Hong Kong/97 (H5N1) viruses. In: Journal of Infectious Diseases. 1999 ; Vol. 179, No. 5. pp. 1132-1138.
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