Recombinant human leptin attenuates stress axis activity in common carp (Cyprinus carpio L.)

Marnix Gorissen, Nicholas J. Bernier, Remy Manuel, Stefan de Gelder, Juriaan R. Metz, Mark O. Huising, Gert Flik

Research output: Contribution to journalArticlepeer-review

48 Scopus citations


Proper functioning of the endocrine stress axis requires communication between the stress axis and other regulatory mechanisms. We here describe an intimate interplay between the stress axis and recombinant human leptin (rhLeptin) in a teleostean fish, the common carp Cyprinus carpio. Restraint stress (by netting up to 96. h) increased plasma cortisol but did not affect hepatic leptin expression. Perifusion of pituitary glands or head kidneys with rhLeptin revealed direct effects of rhLeptin on both tissues. RhLeptin suppresses basal and CRF-induced ACTH-secretion in a rapid and concentration-dependent manner. The rhLeptin effect persisted for over an hour after administration had been terminated. RhLeptin decreases basal interrenal cortisol secretion in vitro, and by doing so attenuates ACTH-stimulated cortisol production; rhLeptin does not affect interrenal ACTH-sensitivity. Our findings show that the endocrine stress axis activity and leptin are inseparably linked in a teleostean fish, a notion relevant to further our insights in the evolution of leptin physiology in vertebrates.

Original languageEnglish (US)
Pages (from-to)75-81
Number of pages7
JournalGeneral and Comparative Endocrinology
Issue number1
StatePublished - Aug 1 2012
Externally publishedYes


  • ACTH
  • Cortisol
  • CRF
  • Leptin
  • Stress axis
  • Teleostean fish

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Endocrinology


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