Recombinant human growth hormone promotes hematopoietic reconstitution after syngeneic bone marrow transplantation in mice

Zhi Gang Tian, Mary Alice Woody, Rui Sun, Lisbeth A. Welniak, Arati Raziuddin, Satoshi Funakoshi, Galia Tsarfaty, Dan L. Longo, William J Murphy

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Recombinant human growth hormone (rhGH) was administered to mice after syngeneic bone marrow transplantation (BMT) to determine its effect on hematopoietic reconstitution. BALB/c mice were given 10 μg intraperitoneal injections of rhGH every other day for a total of 10 injections following syngeneic BMT. Mice that received rhGH exhibited significant increases in total hematopoietic progenitor cell content (colony-forming unit-culture) in both bone marrow and spleen. Erythroid cell progenitor content (burst- forming unit-erythroid) was also significantly increased after rhGH treatment. Analysis of peripheral blood indicated that administration of rhGH resulted in significant increases in the rate of white blood cell and platelet recovery. Granulocyte marker 8C5+ cells were also increased in the bone marrow and spleens of treated mice. Red blood cell, hematocrit, and hemoglobin levels were increased at all time points after rhGH treatment. No significant pathologic effects or weight gain were observed in mice receiving repeated injections of 10 μg rhGH. Thus, rhGH administration after syngeneic BMT promoted multilineage hematopoietic reconstitution and may be of clinical use for accelerating hematopoiesis after autologous BMT.

Original languageEnglish (US)
Pages (from-to)193-199
Number of pages7
JournalStem Cells
Volume16
Issue number3
StatePublished - 1998
Externally publishedYes

Fingerprint

Isogeneic Transplantation
Human Growth Hormone
Bone Marrow Transplantation
Growth Hormone
Erythroid Precursor Cells
Spleen
Bone Marrow
Injections
Autologous Transplantation
Hematopoiesis
Hematopoietic Stem Cells
Intraperitoneal Injections
Hematocrit
Granulocytes
Weight Gain
Hemoglobins
Leukocytes
Stem Cells
Blood Platelets
Erythrocytes

Keywords

  • Bone marrow transplantation
  • Growth hormone
  • Hematopoiesis
  • Neuroendocrine

ASJC Scopus subject areas

  • Cell Biology

Cite this

Tian, Z. G., Woody, M. A., Sun, R., Welniak, L. A., Raziuddin, A., Funakoshi, S., ... Murphy, W. J. (1998). Recombinant human growth hormone promotes hematopoietic reconstitution after syngeneic bone marrow transplantation in mice. Stem Cells, 16(3), 193-199.

Recombinant human growth hormone promotes hematopoietic reconstitution after syngeneic bone marrow transplantation in mice. / Tian, Zhi Gang; Woody, Mary Alice; Sun, Rui; Welniak, Lisbeth A.; Raziuddin, Arati; Funakoshi, Satoshi; Tsarfaty, Galia; Longo, Dan L.; Murphy, William J.

In: Stem Cells, Vol. 16, No. 3, 1998, p. 193-199.

Research output: Contribution to journalArticle

Tian, ZG, Woody, MA, Sun, R, Welniak, LA, Raziuddin, A, Funakoshi, S, Tsarfaty, G, Longo, DL & Murphy, WJ 1998, 'Recombinant human growth hormone promotes hematopoietic reconstitution after syngeneic bone marrow transplantation in mice', Stem Cells, vol. 16, no. 3, pp. 193-199.
Tian ZG, Woody MA, Sun R, Welniak LA, Raziuddin A, Funakoshi S et al. Recombinant human growth hormone promotes hematopoietic reconstitution after syngeneic bone marrow transplantation in mice. Stem Cells. 1998;16(3):193-199.
Tian, Zhi Gang ; Woody, Mary Alice ; Sun, Rui ; Welniak, Lisbeth A. ; Raziuddin, Arati ; Funakoshi, Satoshi ; Tsarfaty, Galia ; Longo, Dan L. ; Murphy, William J. / Recombinant human growth hormone promotes hematopoietic reconstitution after syngeneic bone marrow transplantation in mice. In: Stem Cells. 1998 ; Vol. 16, No. 3. pp. 193-199.
@article{a73e8e35d3ac47bd903dd40ae212549a,
title = "Recombinant human growth hormone promotes hematopoietic reconstitution after syngeneic bone marrow transplantation in mice",
abstract = "Recombinant human growth hormone (rhGH) was administered to mice after syngeneic bone marrow transplantation (BMT) to determine its effect on hematopoietic reconstitution. BALB/c mice were given 10 μg intraperitoneal injections of rhGH every other day for a total of 10 injections following syngeneic BMT. Mice that received rhGH exhibited significant increases in total hematopoietic progenitor cell content (colony-forming unit-culture) in both bone marrow and spleen. Erythroid cell progenitor content (burst- forming unit-erythroid) was also significantly increased after rhGH treatment. Analysis of peripheral blood indicated that administration of rhGH resulted in significant increases in the rate of white blood cell and platelet recovery. Granulocyte marker 8C5+ cells were also increased in the bone marrow and spleens of treated mice. Red blood cell, hematocrit, and hemoglobin levels were increased at all time points after rhGH treatment. No significant pathologic effects or weight gain were observed in mice receiving repeated injections of 10 μg rhGH. Thus, rhGH administration after syngeneic BMT promoted multilineage hematopoietic reconstitution and may be of clinical use for accelerating hematopoiesis after autologous BMT.",
keywords = "Bone marrow transplantation, Growth hormone, Hematopoiesis, Neuroendocrine",
author = "Tian, {Zhi Gang} and Woody, {Mary Alice} and Rui Sun and Welniak, {Lisbeth A.} and Arati Raziuddin and Satoshi Funakoshi and Galia Tsarfaty and Longo, {Dan L.} and Murphy, {William J}",
year = "1998",
language = "English (US)",
volume = "16",
pages = "193--199",
journal = "Stem Cells",
issn = "1066-5099",
publisher = "AlphaMed Press",
number = "3",

}

TY - JOUR

T1 - Recombinant human growth hormone promotes hematopoietic reconstitution after syngeneic bone marrow transplantation in mice

AU - Tian, Zhi Gang

AU - Woody, Mary Alice

AU - Sun, Rui

AU - Welniak, Lisbeth A.

AU - Raziuddin, Arati

AU - Funakoshi, Satoshi

AU - Tsarfaty, Galia

AU - Longo, Dan L.

AU - Murphy, William J

PY - 1998

Y1 - 1998

N2 - Recombinant human growth hormone (rhGH) was administered to mice after syngeneic bone marrow transplantation (BMT) to determine its effect on hematopoietic reconstitution. BALB/c mice were given 10 μg intraperitoneal injections of rhGH every other day for a total of 10 injections following syngeneic BMT. Mice that received rhGH exhibited significant increases in total hematopoietic progenitor cell content (colony-forming unit-culture) in both bone marrow and spleen. Erythroid cell progenitor content (burst- forming unit-erythroid) was also significantly increased after rhGH treatment. Analysis of peripheral blood indicated that administration of rhGH resulted in significant increases in the rate of white blood cell and platelet recovery. Granulocyte marker 8C5+ cells were also increased in the bone marrow and spleens of treated mice. Red blood cell, hematocrit, and hemoglobin levels were increased at all time points after rhGH treatment. No significant pathologic effects or weight gain were observed in mice receiving repeated injections of 10 μg rhGH. Thus, rhGH administration after syngeneic BMT promoted multilineage hematopoietic reconstitution and may be of clinical use for accelerating hematopoiesis after autologous BMT.

AB - Recombinant human growth hormone (rhGH) was administered to mice after syngeneic bone marrow transplantation (BMT) to determine its effect on hematopoietic reconstitution. BALB/c mice were given 10 μg intraperitoneal injections of rhGH every other day for a total of 10 injections following syngeneic BMT. Mice that received rhGH exhibited significant increases in total hematopoietic progenitor cell content (colony-forming unit-culture) in both bone marrow and spleen. Erythroid cell progenitor content (burst- forming unit-erythroid) was also significantly increased after rhGH treatment. Analysis of peripheral blood indicated that administration of rhGH resulted in significant increases in the rate of white blood cell and platelet recovery. Granulocyte marker 8C5+ cells were also increased in the bone marrow and spleens of treated mice. Red blood cell, hematocrit, and hemoglobin levels were increased at all time points after rhGH treatment. No significant pathologic effects or weight gain were observed in mice receiving repeated injections of 10 μg rhGH. Thus, rhGH administration after syngeneic BMT promoted multilineage hematopoietic reconstitution and may be of clinical use for accelerating hematopoiesis after autologous BMT.

KW - Bone marrow transplantation

KW - Growth hormone

KW - Hematopoiesis

KW - Neuroendocrine

UR - http://www.scopus.com/inward/record.url?scp=0031832405&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031832405&partnerID=8YFLogxK

M3 - Article

C2 - 9617894

AN - SCOPUS:0031832405

VL - 16

SP - 193

EP - 199

JO - Stem Cells

JF - Stem Cells

SN - 1066-5099

IS - 3

ER -