Recombinant human BMP-7 effectively prevents non-union in both young and old rats

David J. Hak, Takeshi Makino, Takahiro Niikura, Scott J. Hazelwood, Shane Curtiss, A Hari Reddi

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The purpose of this study was to evaluate the influence of age on the effectiveness of rhBMP-7 treatment in a fracture with severe periosteal damage that is known to result in non-union formation. Closed stabilized femur fractures were produced in 3-month-old and 18-month-old rats. The fracture site was exposed and 2 mm of the periosteum cauterized circumferentially to impair normal fracture healing. The cauterized fracture site was immediately treated with either 100 μg rhBMP-7 (BMP-7 group), or with 25 μL of vehicle alone (control group). Fracture healing was evaluated with radiographs taken at 3 and 6 weeks. Animals were sacrificed at 3 and 6 weeks and specimens subjected to biomechanical and histological evaluation. In both age groups, none of the control animals healed throughout the 6 weeks experimental duration. All of the rhBMP-7-treated 3-month-old animals were radiographically healed at 3 weeks. In comparison, only 56% (9/16) of the rhBMP-7-treated 18-month-old animals were radiographically healed at 3 weeks. At 6 weeks, however, all of the 18-month-old rhBMP-7-treated animals had healed. Histology revealed slower healing in the 18-month-old animals. Treatment with rhBMP-7 significantly increased all of the biomechanical properties in both age groups. In the 3-month-old animals the mechanical strength approached that of the intact femur at 3 weeks, while in the 18-month-old animals this did not occur until 6 weeks. In conclusion, rhBMP-7 can effectively stimulate fracture repair in both young (3-month-old) and old (18-month-old) rats. However, the effect of rhBMP-7 on the rate of fracture healing is greater in young rats compared to old rats.

Original languageEnglish (US)
Pages (from-to)11-20
Number of pages10
JournalJournal of Orthopaedic Research
Volume24
Issue number1
DOIs
StatePublished - Jan 2006

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Bone Morphogenetic Protein 7
Fracture Healing
Femur
Age Groups
Periosteum
Histology
Control Groups

Keywords

  • Aging
  • BMP
  • Fracture repair
  • Non-union

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

Recombinant human BMP-7 effectively prevents non-union in both young and old rats. / Hak, David J.; Makino, Takeshi; Niikura, Takahiro; Hazelwood, Scott J.; Curtiss, Shane; Reddi, A Hari.

In: Journal of Orthopaedic Research, Vol. 24, No. 1, 01.2006, p. 11-20.

Research output: Contribution to journalArticle

Hak, David J. ; Makino, Takeshi ; Niikura, Takahiro ; Hazelwood, Scott J. ; Curtiss, Shane ; Reddi, A Hari. / Recombinant human BMP-7 effectively prevents non-union in both young and old rats. In: Journal of Orthopaedic Research. 2006 ; Vol. 24, No. 1. pp. 11-20.
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