Recombinant hepatitis E capsid protein self-assembles into a dual-domain T = 1 particle presenting native virus epitopes

Li Xing, Kenzo Kato, Tiancheng Li, Naokazu Takeda, Tatsuo Miyamura, Lena Hammar, R. Holland Cheng

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

The three-dimensional structure of a self-assembled, recombinant hepatitis E virus particle has been solved to 22-Å resolution by cryo- electron microscopy and three-dimensional image reconstruction. The single subunit of 50 kDa is derived from a truncated version of the open reading frame-2 gene of the virus expressed in a baculovirus system. This is the first structure of a T = 1 particle with protruding dimers at the icosahedral two-fold axes solved by cryo-electron microscopy. The protein shell of these hollow particles extends from a radius of 50 Å outward to a radius of 135 Å. In the reconstruction, the capsid is dominated by dimers that define the 30 morphological units. The outer domain of the homodimer forms a protrusion, which corresponds to the spike-like density seen in the cryo-electron micrograph. This particle retains native virus epitopes, suggesting its potential value as a vaccine.

Original languageEnglish (US)
Pages (from-to)35-45
Number of pages11
JournalVirology
Volume265
Issue number1
DOIs
StatePublished - Dec 5 1999
Externally publishedYes

Fingerprint

Hepatitis E
Cryoelectron Microscopy
Capsid Proteins
Epitopes
Hepatitis E virus
Viruses
Computer-Assisted Image Processing
Three-Dimensional Imaging
Baculoviridae
Capsid
Virion
Open Reading Frames
Vaccines
Electrons
Genes
Proteins

Keywords

  • Cryo-EM image reconstruction
  • Human hepatitis E virus
  • Quasi- equivalence
  • Recombinant virus-like particle

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Recombinant hepatitis E capsid protein self-assembles into a dual-domain T = 1 particle presenting native virus epitopes. / Xing, Li; Kato, Kenzo; Li, Tiancheng; Takeda, Naokazu; Miyamura, Tatsuo; Hammar, Lena; Cheng, R. Holland.

In: Virology, Vol. 265, No. 1, 05.12.1999, p. 35-45.

Research output: Contribution to journalArticle

Xing, L, Kato, K, Li, T, Takeda, N, Miyamura, T, Hammar, L & Cheng, RH 1999, 'Recombinant hepatitis E capsid protein self-assembles into a dual-domain T = 1 particle presenting native virus epitopes', Virology, vol. 265, no. 1, pp. 35-45. https://doi.org/10.1006/viro.1999.0005
Xing, Li ; Kato, Kenzo ; Li, Tiancheng ; Takeda, Naokazu ; Miyamura, Tatsuo ; Hammar, Lena ; Cheng, R. Holland. / Recombinant hepatitis E capsid protein self-assembles into a dual-domain T = 1 particle presenting native virus epitopes. In: Virology. 1999 ; Vol. 265, No. 1. pp. 35-45.
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