Recombinant DNA probe for serotype-specific identification of bluetongue virus 17.

C. C. de Mattos; C. A. de Mattos; Bennie Osburn; C. A. Dangler; R. Y. Chuang; R. H. Doi

Recombinant DNA probe for serotype-specific identification of bluetongue virus 17.

C. C. de Mattos, C. A. de Mattos, Bennie Osburn, C. A. Dangler, R. Y. Chuang, R. H. Doi

UC Davis School of Medicine

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

The double-stranded RNA genome from 117 field isolates of bluetongue virus (BTV) serotypes 10, 11, 13, and 17 was blotted onto nitrocellulose paper and hybridized with a radioactively labeled cloned copy of DNA genome segment 2 of BTV-17. Viral RNA from BTV prototype strains 2, 10, 11, 13, and 17 were used as controls. The probe hybridized only with the viral RNA from prototype BTV-17 virus and field isolates of BTV-17. There was no cross hybridization with field isolates of BTV serotypes 10, 11, and 13. A complementary DNA probe developed from genes coding for BTV serotype specificity was effectively used in a slot-blot hybridization system for efficiently characterizing the viral serotype.

Original language	English (US)
Pages (from-to)	536-541
Number of pages	6
Journal	American Journal of Veterinary Research
Volume	50
Issue number	4
State	Published - Apr 1 1989

ASJC Scopus subject areas

veterinary(all)

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title = "Recombinant DNA probe for serotype-specific identification of bluetongue virus 17.",

abstract = "The double-stranded RNA genome from 117 field isolates of bluetongue virus (BTV) serotypes 10, 11, 13, and 17 was blotted onto nitrocellulose paper and hybridized with a radioactively labeled cloned copy of DNA genome segment 2 of BTV-17. Viral RNA from BTV prototype strains 2, 10, 11, 13, and 17 were used as controls. The probe hybridized only with the viral RNA from prototype BTV-17 virus and field isolates of BTV-17. There was no cross hybridization with field isolates of BTV serotypes 10, 11, and 13. A complementary DNA probe developed from genes coding for BTV serotype specificity was effectively used in a slot-blot hybridization system for efficiently characterizing the viral serotype.",

author = "{de Mattos}, {C. C.} and {de Mattos}, {C. A.} and Bennie Osburn and Dangler, {C. A.} and Chuang, {R. Y.} and Doi, {R. H.}",

year = "1989",

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language = "English (US)",

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AU - de Mattos, C. C.

AU - de Mattos, C. A.

AU - Osburn, Bennie

AU - Dangler, C. A.

AU - Chuang, R. Y.

AU - Doi, R. H.

PY - 1989/4/1

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AB - The double-stranded RNA genome from 117 field isolates of bluetongue virus (BTV) serotypes 10, 11, 13, and 17 was blotted onto nitrocellulose paper and hybridized with a radioactively labeled cloned copy of DNA genome segment 2 of BTV-17. Viral RNA from BTV prototype strains 2, 10, 11, 13, and 17 were used as controls. The probe hybridized only with the viral RNA from prototype BTV-17 virus and field isolates of BTV-17. There was no cross hybridization with field isolates of BTV serotypes 10, 11, and 13. A complementary DNA probe developed from genes coding for BTV serotype specificity was effectively used in a slot-blot hybridization system for efficiently characterizing the viral serotype.

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