Recombinant DNA probe for serotype-specific identification of bluetongue virus 17.

C. C. de Mattos, C. A. de Mattos, Bennie Osburn, C. A. Dangler, R. Y. Chuang, R. H. Doi

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Abstract

The double-stranded RNA genome from 117 field isolates of bluetongue virus (BTV) serotypes 10, 11, 13, and 17 was blotted onto nitrocellulose paper and hybridized with a radioactively labeled cloned copy of DNA genome segment 2 of BTV-17. Viral RNA from BTV prototype strains 2, 10, 11, 13, and 17 were used as controls. The probe hybridized only with the viral RNA from prototype BTV-17 virus and field isolates of BTV-17. There was no cross hybridization with field isolates of BTV serotypes 10, 11, and 13. A complementary DNA probe developed from genes coding for BTV serotype specificity was effectively used in a slot-blot hybridization system for efficiently characterizing the viral serotype.

Original languageEnglish (US)
Pages (from-to)536-541
Number of pages6
JournalAmerican Journal of Veterinary Research
Volume50
Issue number4
StatePublished - Apr 1 1989

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ASJC Scopus subject areas

  • veterinary(all)

Cite this

de Mattos, C. C., de Mattos, C. A., Osburn, B., Dangler, C. A., Chuang, R. Y., & Doi, R. H. (1989). Recombinant DNA probe for serotype-specific identification of bluetongue virus 17. American Journal of Veterinary Research, 50(4), 536-541.