Recombinant DNA probe for serotype-specific identification of bluetongue virus 17.

C. C. de Mattos, C. A. de Mattos, Bennie Osburn, C. A. Dangler, R. Y. Chuang, R. H. Doi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The double-stranded RNA genome from 117 field isolates of bluetongue virus (BTV) serotypes 10, 11, 13, and 17 was blotted onto nitrocellulose paper and hybridized with a radioactively labeled cloned copy of DNA genome segment 2 of BTV-17. Viral RNA from BTV prototype strains 2, 10, 11, 13, and 17 were used as controls. The probe hybridized only with the viral RNA from prototype BTV-17 virus and field isolates of BTV-17. There was no cross hybridization with field isolates of BTV serotypes 10, 11, and 13. A complementary DNA probe developed from genes coding for BTV serotype specificity was effectively used in a slot-blot hybridization system for efficiently characterizing the viral serotype.

Original languageEnglish (US)
Pages (from-to)536-541
Number of pages6
JournalAmerican Journal of Veterinary Research
Volume50
Issue number4
StatePublished - Apr 1 1989

Fingerprint

Bluetongue virus
recombinant DNA
Recombinant DNA
DNA probes
DNA Probes
serotypes
Viral RNA
prototypes
hybridization
Genome
RNA
complementary DNA
Collodion
genome
Double-Stranded RNA
RNA Viruses
double-stranded RNA
Serogroup
Complementary DNA
Viruses

ASJC Scopus subject areas

  • veterinary(all)

Cite this

de Mattos, C. C., de Mattos, C. A., Osburn, B., Dangler, C. A., Chuang, R. Y., & Doi, R. H. (1989). Recombinant DNA probe for serotype-specific identification of bluetongue virus 17. American Journal of Veterinary Research, 50(4), 536-541.

Recombinant DNA probe for serotype-specific identification of bluetongue virus 17. / de Mattos, C. C.; de Mattos, C. A.; Osburn, Bennie; Dangler, C. A.; Chuang, R. Y.; Doi, R. H.

In: American Journal of Veterinary Research, Vol. 50, No. 4, 01.04.1989, p. 536-541.

Research output: Contribution to journalArticle

de Mattos, CC, de Mattos, CA, Osburn, B, Dangler, CA, Chuang, RY & Doi, RH 1989, 'Recombinant DNA probe for serotype-specific identification of bluetongue virus 17.', American Journal of Veterinary Research, vol. 50, no. 4, pp. 536-541.
de Mattos CC, de Mattos CA, Osburn B, Dangler CA, Chuang RY, Doi RH. Recombinant DNA probe for serotype-specific identification of bluetongue virus 17. American Journal of Veterinary Research. 1989 Apr 1;50(4):536-541.
de Mattos, C. C. ; de Mattos, C. A. ; Osburn, Bennie ; Dangler, C. A. ; Chuang, R. Y. ; Doi, R. H. / Recombinant DNA probe for serotype-specific identification of bluetongue virus 17. In: American Journal of Veterinary Research. 1989 ; Vol. 50, No. 4. pp. 536-541.
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