Abstract
The double-stranded RNA genome from 117 field isolates of bluetongue virus (BTV) serotypes 10, 11, 13, and 17 was blotted onto nitrocellulose paper and hybridized with a radioactively labeled cloned copy of DNA genome segment 2 of BTV-17. Viral RNA from BTV prototype strains 2, 10, 11, 13, and 17 were used as controls. The probe hybridized only with the viral RNA from prototype BTV-17 virus and field isolates of BTV-17. There was no cross hybridization with field isolates of BTV serotypes 10, 11, and 13. A complementary DNA probe developed from genes coding for BTV serotype specificity was effectively used in a slot-blot hybridization system for efficiently characterizing the viral serotype.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 536-541 |
| Number of pages | 6 |
| Journal | American Journal of Veterinary Research |
| Volume | 50 |
| Issue number | 4 |
| State | Published - Apr 1 1989 |
ASJC Scopus subject areas
- veterinary(all)
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Cite this
de Mattos, C. C., de Mattos, C. A., Osburn, B., Dangler, C. A., Chuang, R. Y., & Doi, R. H. (1989). Recombinant DNA probe for serotype-specific identification of bluetongue virus 17. American Journal of Veterinary Research, 50(4), 536-541.