Recombinant cell bioassays for endocrine disruptors: Development of a stably transfected human ovarian cell line for the detection of estrogenic and anti-estrogenic chemicals

Jane M. Rogers, Michael S. Denison

Research output: Contribution to journalArticle

113 Scopus citations

Abstract

The ability of a variety of compounds to disrupt normal endocrine homeostasis, and potentially, the physiological and reproductive capacity of an organism, has gained worldwide attention in recent years. In an attempt to identify such compounds, and to examine the mechanism(s) by which they may exert their actions, we have constructed reporter plasmid vectors that contain the firefly luciferase gene under hormone-inducible control of estrogen-, androgen-, or retinoic acid-responsive DNA enhancer elements. Transient transfection of these vectors into appropriate receptor-containing cell lines revealed their ability to respond to their respective hormones with the induction of luciferase. Here, we describe development and optimization of a recombinant human ovarian carcinoma (BG1) line, which has been stably transfected with the estrogen responsive luciferase reporter plasmid. The resulting recombinant cell line (BG1Luc4E2) responds to 17β- estradiol at concentrations as low as 1 pM. The utility of BG1Luc4E2 cells as a bioassay screening system for environmental estrogens was demonstrated by their response to known xenoestrogens, and also by the putative identification of two polychlorinated biphenyls (2,3',4,4,'- tetrachlorobiphenyl and 2,2',3,5',6-pentachlorobiphenyl) as novel estrogenic chemicals. These cell bioassay systems have applications for rapid screening, identification, and characterization of endocrine disrupting chemicals.

Original languageEnglish (US)
Pages (from-to)67-82
Number of pages16
JournalIn Vitro and Molecular Toxicology: Journal of Basic and Applied Research
Volume13
Issue number1
StatePublished - Mar 2000

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ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

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