Abstract
Genome segment 10 of bluetongue virus (BTV) serotype 11 UC8 strain was cloned and subsequently hybridized to viral double-stranded RNA extracted from 90 field isolates of BTV serotypes 10, 11, 13, and 17; the prototype strains of BTV 2, 10, 11, 13, and 17; the prototype strain epizootic hemorrhagic disease virus (EHDV) serotype 1; and 4 field isolates of EHDV serotype 2. The 90 field isolates were obtained from different counties in California, Louisiana, and Idaho during the years 1979, 1980, and 1981. The cloned genetic probe hybridized with all the BTV samples tested, showing different degrees of cross-hybridization at the stringency conditions used in this study. This indicated that BTV genome segment 10 has conserved nucleotide sequences among the BTV serotypes 2, 10, 11, 13, and 17. No cross-hybridization signals were detected between the cloned genome segment 10 of BTV 11 UC8 strain and the prototype strain of EHDV serotype 1 and the field isolates of serotype 2. This probe recognized a wide variety of BTV isolates.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 237-241 |
| Number of pages | 5 |
| Journal | Journal of Veterinary Diagnostic Investigation |
| Volume | 1 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jan 1 1989 |
Fingerprint
ASJC Scopus subject areas
- veterinary(all)
Cite this
Recombinant cDNA probe from bluetongue virus genome segment 10 for identification of bluetongue virus. / de Mattos, Carlos Alberto; de Mattos, Cecilia Cristina; Osburn, Bennie.
In: Journal of Veterinary Diagnostic Investigation, Vol. 1, No. 3, 01.01.1989, p. 237-241.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Recombinant cDNA probe from bluetongue virus genome segment 10 for identification of bluetongue virus
AU - de Mattos, Carlos Alberto
AU - de Mattos, Cecilia Cristina
AU - Osburn, Bennie
PY - 1989/1/1
Y1 - 1989/1/1
N2 - Genome segment 10 of bluetongue virus (BTV) serotype 11 UC8 strain was cloned and subsequently hybridized to viral double-stranded RNA extracted from 90 field isolates of BTV serotypes 10, 11, 13, and 17; the prototype strains of BTV 2, 10, 11, 13, and 17; the prototype strain epizootic hemorrhagic disease virus (EHDV) serotype 1; and 4 field isolates of EHDV serotype 2. The 90 field isolates were obtained from different counties in California, Louisiana, and Idaho during the years 1979, 1980, and 1981. The cloned genetic probe hybridized with all the BTV samples tested, showing different degrees of cross-hybridization at the stringency conditions used in this study. This indicated that BTV genome segment 10 has conserved nucleotide sequences among the BTV serotypes 2, 10, 11, 13, and 17. No cross-hybridization signals were detected between the cloned genome segment 10 of BTV 11 UC8 strain and the prototype strain of EHDV serotype 1 and the field isolates of serotype 2. This probe recognized a wide variety of BTV isolates.
AB - Genome segment 10 of bluetongue virus (BTV) serotype 11 UC8 strain was cloned and subsequently hybridized to viral double-stranded RNA extracted from 90 field isolates of BTV serotypes 10, 11, 13, and 17; the prototype strains of BTV 2, 10, 11, 13, and 17; the prototype strain epizootic hemorrhagic disease virus (EHDV) serotype 1; and 4 field isolates of EHDV serotype 2. The 90 field isolates were obtained from different counties in California, Louisiana, and Idaho during the years 1979, 1980, and 1981. The cloned genetic probe hybridized with all the BTV samples tested, showing different degrees of cross-hybridization at the stringency conditions used in this study. This indicated that BTV genome segment 10 has conserved nucleotide sequences among the BTV serotypes 2, 10, 11, 13, and 17. No cross-hybridization signals were detected between the cloned genome segment 10 of BTV 11 UC8 strain and the prototype strain of EHDV serotype 1 and the field isolates of serotype 2. This probe recognized a wide variety of BTV isolates.
UR - http://www.scopus.com/inward/record.url?scp=0024702719&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024702719&partnerID=8YFLogxK
U2 - 10.1177/104063878900100308
DO - 10.1177/104063878900100308
M3 - Article
C2 - 2562196
AN - SCOPUS:0024702719
VL - 1
SP - 237
EP - 241
JO - Journal of Veterinary Diagnostic Investigation
JF - Journal of Veterinary Diagnostic Investigation
SN - 1040-6387
IS - 3
ER -