Recombinant cDNA probe from bluetongue virus genome segment 10 for identification of bluetongue virus

Carlos Alberto de Mattos, Cecilia Cristina de Mattos, Bennie Osburn

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Genome segment 10 of bluetongue virus (BTV) serotype 11 UC8 strain was cloned and subsequently hybridized to viral double-stranded RNA extracted from 90 field isolates of BTV serotypes 10, 11, 13, and 17; the prototype strains of BTV 2, 10, 11, 13, and 17; the prototype strain epizootic hemorrhagic disease virus (EHDV) serotype 1; and 4 field isolates of EHDV serotype 2. The 90 field isolates were obtained from different counties in California, Louisiana, and Idaho during the years 1979, 1980, and 1981. The cloned genetic probe hybridized with all the BTV samples tested, showing different degrees of cross-hybridization at the stringency conditions used in this study. This indicated that BTV genome segment 10 has conserved nucleotide sequences among the BTV serotypes 2, 10, 11, 13, and 17. No cross-hybridization signals were detected between the cloned genome segment 10 of BTV 11 UC8 strain and the prototype strain of EHDV serotype 1 and the field isolates of serotype 2. This probe recognized a wide variety of BTV isolates.

Original languageEnglish (US)
Pages (from-to)237-241
Number of pages5
JournalJournal of Veterinary Diagnostic Investigation
Volume1
Issue number3
DOIs
StatePublished - Jan 1 1989

Fingerprint

Bluetongue virus
Complementary DNA
serotypes
Genome
Epizootic hemorrhagic disease virus
genome
prototypes
hybridization
Double-Stranded RNA
Conserved Sequence
double-stranded RNA
Viral RNA
Serogroup
nucleotide sequences

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Recombinant cDNA probe from bluetongue virus genome segment 10 for identification of bluetongue virus. / de Mattos, Carlos Alberto; de Mattos, Cecilia Cristina; Osburn, Bennie.

In: Journal of Veterinary Diagnostic Investigation, Vol. 1, No. 3, 01.01.1989, p. 237-241.

Research output: Contribution to journalArticle

de Mattos, Carlos Alberto ; de Mattos, Cecilia Cristina ; Osburn, Bennie. / Recombinant cDNA probe from bluetongue virus genome segment 10 for identification of bluetongue virus. In: Journal of Veterinary Diagnostic Investigation. 1989 ; Vol. 1, No. 3. pp. 237-241.
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