Abstract
We describe the development and preliminary characterization of a recombinant canarypox virus vectored vaccine for protective immunization of ruminants against bluetongue virus (BTV) infection. Sheep (n = 6) immunized with recombinant canarypox virus vector (BTV-CP) co-expressing synthetic genes encoding the two outer capsid proteins (VP2 and VP5) of BTV serotype 17 (BTV-17) developed high titers (40-160) of virus-specific neutralizing antibodies and were resistant to challenge with a field strain of BTV-17. In contrast, sheep (n = 5) immunized with a commercial recombinant canarypox virus vector expressing the E and preM genes of West Nile virus were seronegative to BTV and developed pyrexia, lymphopenia, and extended, high-titered viremias following challenge exposure to the field strain of BTV-17. These data confirm that the BTV-CP vaccine may be useful for the protective immunization of ruminants against bluetongue, and it may avoid the problems inherent to live-attenuated (LA) BTV vaccines.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 672-678 |
| Number of pages | 7 |
| Journal | Vaccine |
| Volume | 25 |
| Issue number | 4 |
| DOIs | |
| State | Published - Jan 8 2007 |
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Keywords
- Bluetongue
- Canarypox virus
- Sheep
- Vaccine
ASJC Scopus subject areas
- Immunology
- Microbiology
- Virology
- Infectious Diseases
- Public Health, Environmental and Occupational Health
- veterinary(all)
Cite this
Recombinant canarypox virus vaccine co-expressing genes encoding the VP2 and VP5 outer capsid proteins of bluetongue virus induces high level protection in sheep. / Boone, Josh D.; Balasuriya, Udeni B.; Karaca, Kemal; Audonnet, Jean Christophe; Yao, Jiansheng; He, Ling; Nordgren, Robert; Monaco, Federica; Savini, Giovanni; Gardner, Ian; Maclachlan, Nigel J.
In: Vaccine, Vol. 25, No. 4, 08.01.2007, p. 672-678.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Recombinant canarypox virus vaccine co-expressing genes encoding the VP2 and VP5 outer capsid proteins of bluetongue virus induces high level protection in sheep
AU - Boone, Josh D.
AU - Balasuriya, Udeni B.
AU - Karaca, Kemal
AU - Audonnet, Jean Christophe
AU - Yao, Jiansheng
AU - He, Ling
AU - Nordgren, Robert
AU - Monaco, Federica
AU - Savini, Giovanni
AU - Gardner, Ian
AU - Maclachlan, Nigel J
PY - 2007/1/8
Y1 - 2007/1/8
N2 - We describe the development and preliminary characterization of a recombinant canarypox virus vectored vaccine for protective immunization of ruminants against bluetongue virus (BTV) infection. Sheep (n = 6) immunized with recombinant canarypox virus vector (BTV-CP) co-expressing synthetic genes encoding the two outer capsid proteins (VP2 and VP5) of BTV serotype 17 (BTV-17) developed high titers (40-160) of virus-specific neutralizing antibodies and were resistant to challenge with a field strain of BTV-17. In contrast, sheep (n = 5) immunized with a commercial recombinant canarypox virus vector expressing the E and preM genes of West Nile virus were seronegative to BTV and developed pyrexia, lymphopenia, and extended, high-titered viremias following challenge exposure to the field strain of BTV-17. These data confirm that the BTV-CP vaccine may be useful for the protective immunization of ruminants against bluetongue, and it may avoid the problems inherent to live-attenuated (LA) BTV vaccines.
AB - We describe the development and preliminary characterization of a recombinant canarypox virus vectored vaccine for protective immunization of ruminants against bluetongue virus (BTV) infection. Sheep (n = 6) immunized with recombinant canarypox virus vector (BTV-CP) co-expressing synthetic genes encoding the two outer capsid proteins (VP2 and VP5) of BTV serotype 17 (BTV-17) developed high titers (40-160) of virus-specific neutralizing antibodies and were resistant to challenge with a field strain of BTV-17. In contrast, sheep (n = 5) immunized with a commercial recombinant canarypox virus vector expressing the E and preM genes of West Nile virus were seronegative to BTV and developed pyrexia, lymphopenia, and extended, high-titered viremias following challenge exposure to the field strain of BTV-17. These data confirm that the BTV-CP vaccine may be useful for the protective immunization of ruminants against bluetongue, and it may avoid the problems inherent to live-attenuated (LA) BTV vaccines.
KW - Bluetongue
KW - Canarypox virus
KW - Sheep
KW - Vaccine
UR - http://www.scopus.com/inward/record.url?scp=33845344012&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33845344012&partnerID=8YFLogxK
U2 - 10.1016/j.vaccine.2006.08.025
DO - 10.1016/j.vaccine.2006.08.025
M3 - Article
C2 - 17059856
AN - SCOPUS:33845344012
VL - 25
SP - 672
EP - 678
JO - Vaccine
JF - Vaccine
SN - 0264-410X
IS - 4
ER -