Recombinant canarypox virus vaccine co-expressing genes encoding the VP2 and VP5 outer capsid proteins of bluetongue virus induces high level protection in sheep

Josh D. Boone, Udeni B. Balasuriya, Kemal Karaca, Jean Christophe Audonnet, Jiansheng Yao, Ling He, Robert Nordgren, Federica Monaco, Giovanni Savini, Ian Gardner, Nigel J Maclachlan

Research output: Contribution to journalArticle

76 Scopus citations

Abstract

We describe the development and preliminary characterization of a recombinant canarypox virus vectored vaccine for protective immunization of ruminants against bluetongue virus (BTV) infection. Sheep (n = 6) immunized with recombinant canarypox virus vector (BTV-CP) co-expressing synthetic genes encoding the two outer capsid proteins (VP2 and VP5) of BTV serotype 17 (BTV-17) developed high titers (40-160) of virus-specific neutralizing antibodies and were resistant to challenge with a field strain of BTV-17. In contrast, sheep (n = 5) immunized with a commercial recombinant canarypox virus vector expressing the E and preM genes of West Nile virus were seronegative to BTV and developed pyrexia, lymphopenia, and extended, high-titered viremias following challenge exposure to the field strain of BTV-17. These data confirm that the BTV-CP vaccine may be useful for the protective immunization of ruminants against bluetongue, and it may avoid the problems inherent to live-attenuated (LA) BTV vaccines.

Original languageEnglish (US)
Pages (from-to)672-678
Number of pages7
JournalVaccine
Volume25
Issue number4
DOIs
StatePublished - Jan 8 2007

Keywords

  • Bluetongue
  • Canarypox virus
  • Sheep
  • Vaccine

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

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