Recognition of the alternatively spliced segments of fibronectin by the RCJ 3.1C5.18 chondrocytic rat cell line

C. Fernandez, S. Jami, G. Loredo, F. Ko, T. Hahn, S. McDougall, J. H. Peters

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Objectives: To define, for the C5.18 chondrocyte-restricted rat cell line, (1) the capacities for recognition of alternatively spliced segments of the adhesion protein fibronectin (FN), (2) the integrin subunits required for such recognition, and (3) differences in such FN recognition vs the multipotential chondroprogenitor line, RCJ 3.1. Methods: C5.18 and RCJ 3.1 cells were tested for their capacities to adhere to recombinant alternatively spliced segments of rat FN, presented on plastic surfaces either in isolation or in partial FNs spanning the 7th through 15th type III repeats (III7-15 FNs). The effects on such adhesion of cations and integrin subunit-specific antibodies were tested. Results: Despite significant augmentation in chondrocyte-specific gene expression in C5.18 relative to the RCJ 3.1 cells, the two lines exhibited similar recognition of FN spliced segments and partial isoforms. Specifically, both lines adhered to the extra type III repeat A (EIIIA) and V, but not extra type III repeat B (EIIIB), segments. There were different cation and integrin subunit requirements for adhesion to EIIIA vs V segments, and only the V segment was recognized in the context of a III7-15 FN. Such recognition was mediated via a "second" arginine-glycine-aspartic acid (RGD) sequence that is present in the V95 subsegment in rat, but not human, FN. Conclusion: The chondrocyte lineage-committed C5.18 cell line, similar to its multipotential chondroprogenitor, RCJ 3.1, recognizes the "cartilage-restricted" EIIIA and V segments of FN with cation, integrin, and molecular context requirements that are specific to each of these segments.

Original languageEnglish (US)
Pages (from-to)228-239
Number of pages12
JournalOsteoarthritis and Cartilage
Volume18
Issue number2
DOIs
StatePublished - Feb 2010

Fingerprint

Fibronectins
Rats
Adhesion
Positive ions
Cells
Cell Line
Integrins
Chondrocytes
Arginine
Cations
Cartilage
Gene expression
Antibodies
Amino acids
Plastics
Proteins
Acids
Aspartic Acid
Glycine
Protein Isoforms

Keywords

  • Cartilage
  • Chondrocyte
  • Differentiation
  • Fibronectin alternative splicing
  • Mulipotential stem cell

ASJC Scopus subject areas

  • Biomedical Engineering
  • Orthopedics and Sports Medicine
  • Rheumatology

Cite this

Recognition of the alternatively spliced segments of fibronectin by the RCJ 3.1C5.18 chondrocytic rat cell line. / Fernandez, C.; Jami, S.; Loredo, G.; Ko, F.; Hahn, T.; McDougall, S.; Peters, J. H.

In: Osteoarthritis and Cartilage, Vol. 18, No. 2, 02.2010, p. 228-239.

Research output: Contribution to journalArticle

Fernandez, C. ; Jami, S. ; Loredo, G. ; Ko, F. ; Hahn, T. ; McDougall, S. ; Peters, J. H. / Recognition of the alternatively spliced segments of fibronectin by the RCJ 3.1C5.18 chondrocytic rat cell line. In: Osteoarthritis and Cartilage. 2010 ; Vol. 18, No. 2. pp. 228-239.
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