Recognition of multivalent histone states associated with heterochromatin by UHRF1 protein

Nataliya Nady, Alexander Lemak, John R. Walker, George V. Avvakumov, Michael S. Kareta, Mayada Achour, Sheng Xue, Shili Duan, Abdellah Allali-Hassani, Xiaobing Zuo, Yun Xing Wang, Christian Bronner, Frederic Chedin, Cheryl H. Arrowsmith, Sirano Dhe-Paganon

Research output: Contribution to journalArticle

109 Citations (Scopus)

Abstract

Histone modifications and DNA methylation represent two layers of heritable epigenetic information that regulate eukaryotic chromatin structure and gene activity. UHRF1 is a unique factor that bridges these two layers; it is required for maintenance DNA methylation at hemimethylated CpG sites, which are specifically recognized through its SRA domain and also interacts with histone H3 trimethylated on lysine 9 (H3K9me3) in an unspecified manner. Here we show that UHRF1 contains a tandem Tudor domain (TTD) that recognizes H3 tail peptides with the heterochromatin-associated modification state of trimethylated lysine 9 and unmodified lysine 4 (H3K4me0/K9me3). Solution NMR and crystallographic data reveal the TTD simultaneously recognizes H3K9me3 through a conserved aromatic cage in the first Tudor subdomain and unmodified H3K4 within a groove between the tandem subdomains. The subdomains undergo a conformational adjustment upon peptide binding, distinct from previously reported mechanisms for dual histone mark recognition. Mutant UHRF1 protein deficient for H3K4me0/K9me3 binding shows altered localization to heterochromatic chromocenters and fails to reduce expression of a target gene, p16 INK4A, when overexpressed. Our results demonstrate a novel recognition mechanism for the combinatorial readout of histone modification states associated with gene silencing and add to the growing evidence for coordination of, and cross-talk between, the modification states of H3K4 and H3K9 in regulation of gene expression.

Original languageEnglish (US)
Pages (from-to)24300-24311
Number of pages12
JournalJournal of Biological Chemistry
Volume286
Issue number27
DOIs
StatePublished - Jul 8 2011

Fingerprint

Histone Code
Heterochromatin
Histones
Lysine
DNA Methylation
Genes
Cyclin-Dependent Kinase Inhibitor p16
Peptides
Proteins
Gene Expression Regulation
Gene Silencing
Mutant Proteins
Epigenomics
Chromatin
Maintenance
Gene expression
Nuclear magnetic resonance
Tudor Domain

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Nady, N., Lemak, A., Walker, J. R., Avvakumov, G. V., Kareta, M. S., Achour, M., ... Dhe-Paganon, S. (2011). Recognition of multivalent histone states associated with heterochromatin by UHRF1 protein. Journal of Biological Chemistry, 286(27), 24300-24311. https://doi.org/10.1074/jbc.M111.234104

Recognition of multivalent histone states associated with heterochromatin by UHRF1 protein. / Nady, Nataliya; Lemak, Alexander; Walker, John R.; Avvakumov, George V.; Kareta, Michael S.; Achour, Mayada; Xue, Sheng; Duan, Shili; Allali-Hassani, Abdellah; Zuo, Xiaobing; Wang, Yun Xing; Bronner, Christian; Chedin, Frederic; Arrowsmith, Cheryl H.; Dhe-Paganon, Sirano.

In: Journal of Biological Chemistry, Vol. 286, No. 27, 08.07.2011, p. 24300-24311.

Research output: Contribution to journalArticle

Nady, N, Lemak, A, Walker, JR, Avvakumov, GV, Kareta, MS, Achour, M, Xue, S, Duan, S, Allali-Hassani, A, Zuo, X, Wang, YX, Bronner, C, Chedin, F, Arrowsmith, CH & Dhe-Paganon, S 2011, 'Recognition of multivalent histone states associated with heterochromatin by UHRF1 protein', Journal of Biological Chemistry, vol. 286, no. 27, pp. 24300-24311. https://doi.org/10.1074/jbc.M111.234104
Nady, Nataliya ; Lemak, Alexander ; Walker, John R. ; Avvakumov, George V. ; Kareta, Michael S. ; Achour, Mayada ; Xue, Sheng ; Duan, Shili ; Allali-Hassani, Abdellah ; Zuo, Xiaobing ; Wang, Yun Xing ; Bronner, Christian ; Chedin, Frederic ; Arrowsmith, Cheryl H. ; Dhe-Paganon, Sirano. / Recognition of multivalent histone states associated with heterochromatin by UHRF1 protein. In: Journal of Biological Chemistry. 2011 ; Vol. 286, No. 27. pp. 24300-24311.
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