RecBCD enzyme is a bipolar DNA helicase

Mark S. Dillingham, Maria Spies, Stephen C. Kowalczykowski

Research output: Contribution to journalArticle

162 Citations (Scopus)

Abstract

Escherichia coli RecBCD is a heterotrimeric helicase/nuclease that catalyses a complex reaction in which double-strand breaks in DNA are processed for repair by homologous recombination. For some time it has been clear that the RecB subunit possesses a 3′ → 5′ DNA helicase activity, which was thought to drive DNA translocation and unwinding in the RecBCD holoenzyme. Here we show that purified RecD protein is also a DNA helicase, but one that possesses a 5′ → 3′ polarity. We also show that the RecB and RecD helicases are both active in intact RecBCD, because the enzyme remains capable of processive DNA unwinding when either of these subunits is inactivated by mutation. These findings point to a bipolar translocation model for RecBCD in which the two DNA helicases are complementary, travelling with opposite polarities, but in the same direction, on each strand of the antiparallel DNA duplex. This bipolar motor organization helps to explain various biochemical properties of RecBCD, notably its exceptionally high speed and processivity, and offers a mechanistic insight into aspects of RecBCD function.

Original languageEnglish (US)
Pages (from-to)893-897
Number of pages5
JournalNature
Volume423
Issue number6942
DOIs
StatePublished - Jun 19 2003

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Exodeoxyribonuclease V
DNA Helicases
DNA
Recombinational DNA Repair
Holoenzymes
Double-Stranded DNA Breaks
Escherichia coli
Mutation
Proteins

ASJC Scopus subject areas

  • General

Cite this

Dillingham, M. S., Spies, M., & Kowalczykowski, S. C. (2003). RecBCD enzyme is a bipolar DNA helicase. Nature, 423(6942), 893-897. https://doi.org/10.1038/nature01673

RecBCD enzyme is a bipolar DNA helicase. / Dillingham, Mark S.; Spies, Maria; Kowalczykowski, Stephen C.

In: Nature, Vol. 423, No. 6942, 19.06.2003, p. 893-897.

Research output: Contribution to journalArticle

Dillingham, MS, Spies, M & Kowalczykowski, SC 2003, 'RecBCD enzyme is a bipolar DNA helicase', Nature, vol. 423, no. 6942, pp. 893-897. https://doi.org/10.1038/nature01673
Dillingham MS, Spies M, Kowalczykowski SC. RecBCD enzyme is a bipolar DNA helicase. Nature. 2003 Jun 19;423(6942):893-897. https://doi.org/10.1038/nature01673
Dillingham, Mark S. ; Spies, Maria ; Kowalczykowski, Stephen C. / RecBCD enzyme is a bipolar DNA helicase. In: Nature. 2003 ; Vol. 423, No. 6942. pp. 893-897.
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