Abstract
A metabolic pathway engineered Escherichia coli strain (superbug) containing one plasmid harboring an artificial gene cluster encoding all the five enzymes in the biosynthetic pathway of Galα1,3Lac through galactose metabolism has been developed. The plasmid contains a λ promoter, a cl857 repressor gene, an ampicillin resistance gene, and a T7 terminator. Each gene was preceded by a Shine-Dalgarno sequence for ribosome binding. In a reaction catalyzed by the recombinant E. coli strain, Galα1,3Lac trisaccharide accumulated at concentrations of 14.2 mM (7.2 g L-1) in a reaction mixture containing galactose, glucose, lactose, and a catalytic amount of uridine 5′-diphosphoglucose. This work demonstrates that large-scale synthesis of complex oligosaccharides can be achieved economically and efficiently through a single, biosynthetic pathway engineered microorganism.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 47-53 |
| Number of pages | 7 |
| Journal | ChemBioChem |
| Volume | 3 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 4 2002 |
| Externally published | Yes |
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Keywords
- Biosynthesis
- Gene expression
- Glycosylation
- Metabolic engineering
- Oligosaccharides
ASJC Scopus subject areas
- Drug Discovery
- Organic Chemistry
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Reassembled biosynthetic pathway for large-scale carbohydrate synthesis : α-Gal epitope producing "superbug". / Chen, Xi; Liu, Ziye; Zhang, Jianbo; Zhang, Wei; Kowal, Przemyslaw; Wang, Peng George.
In: ChemBioChem, Vol. 3, No. 1, 04.01.2002, p. 47-53.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Reassembled biosynthetic pathway for large-scale carbohydrate synthesis
T2 - α-Gal epitope producing "superbug"
AU - Chen, Xi
AU - Liu, Ziye
AU - Zhang, Jianbo
AU - Zhang, Wei
AU - Kowal, Przemyslaw
AU - Wang, Peng George
PY - 2002/1/4
Y1 - 2002/1/4
N2 - A metabolic pathway engineered Escherichia coli strain (superbug) containing one plasmid harboring an artificial gene cluster encoding all the five enzymes in the biosynthetic pathway of Galα1,3Lac through galactose metabolism has been developed. The plasmid contains a λ promoter, a cl857 repressor gene, an ampicillin resistance gene, and a T7 terminator. Each gene was preceded by a Shine-Dalgarno sequence for ribosome binding. In a reaction catalyzed by the recombinant E. coli strain, Galα1,3Lac trisaccharide accumulated at concentrations of 14.2 mM (7.2 g L-1) in a reaction mixture containing galactose, glucose, lactose, and a catalytic amount of uridine 5′-diphosphoglucose. This work demonstrates that large-scale synthesis of complex oligosaccharides can be achieved economically and efficiently through a single, biosynthetic pathway engineered microorganism.
AB - A metabolic pathway engineered Escherichia coli strain (superbug) containing one plasmid harboring an artificial gene cluster encoding all the five enzymes in the biosynthetic pathway of Galα1,3Lac through galactose metabolism has been developed. The plasmid contains a λ promoter, a cl857 repressor gene, an ampicillin resistance gene, and a T7 terminator. Each gene was preceded by a Shine-Dalgarno sequence for ribosome binding. In a reaction catalyzed by the recombinant E. coli strain, Galα1,3Lac trisaccharide accumulated at concentrations of 14.2 mM (7.2 g L-1) in a reaction mixture containing galactose, glucose, lactose, and a catalytic amount of uridine 5′-diphosphoglucose. This work demonstrates that large-scale synthesis of complex oligosaccharides can be achieved economically and efficiently through a single, biosynthetic pathway engineered microorganism.
KW - Biosynthesis
KW - Gene expression
KW - Glycosylation
KW - Metabolic engineering
KW - Oligosaccharides
UR - http://www.scopus.com/inward/record.url?scp=0037016633&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037016633&partnerID=8YFLogxK
U2 - 10.1002/1439-7633(20020104)3:1<47::AID-CBIC47>3.0.CO;2-N
DO - 10.1002/1439-7633(20020104)3:1<47::AID-CBIC47>3.0.CO;2-N
M3 - Article
C2 - 17590953
AN - SCOPUS:0037016633
VL - 3
SP - 47
EP - 53
JO - ChemBioChem
JF - ChemBioChem
SN - 1439-4227
IS - 1
ER -