Rearrangement of the bacterial chromosome: Forbidden inversions

Anca Segall, Michael J. Mahan, John R. Roth

Research output: Contribution to journalArticlepeer-review

70 Scopus citations

Abstract

The order of genes in the chromosome of enteric bacteria has been evolutionarily conserved despite the existence of mechanisms for rearrangement. Homologous chromosomal sequences in the same orientation recombine to form deletions or duplications. When homologous sequences in inverse orientation recombine, one expects to form an inversion of the intervening chromosomal segment. This expectation was tested by placing pairs of homologous sequences in inverse order at various points in the chromosome. Sequences at many pairs of sites (permissive) do recombine to generate the expected inversion, while the same sequences placed at other pairs of sites (nonpermissive) do not form an inversion. For the one nonpermissive interval tested, the missing inversion type can be constructed by an alternative transductional method; strains with this inversion are viable. Thus mechanistic limitations must prevent sequences at particular sites from undergoing the recombination event required to form an inversion.

Original languageEnglish (US)
Pages (from-to)1314-1318
Number of pages5
JournalScience
Volume241
Issue number4871
StatePublished - 1988
Externally publishedYes

ASJC Scopus subject areas

  • General

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