Real-time whole-plant dynamics of heavy metal transport in Arabidopsis halleri and Arabidopsis thaliana by gamma-ray imaging

Kaisa Kajala, Katherine L. Walker, Gregory Mitchell, Ute Krämer, Simon R Cherry, Siobhan M. Brady

Research output: Contribution to journalArticle

Abstract

Heavy metals such as zinc are essential for plant growth, but toxic at high concentrations. Despite our knowledge of the molecular mechanisms of heavy metal uptake by plants, experimentally addressing the real-time whole-plant dynamics of heavy metal uptake and partitioning has remained a challenge. To overcome this, we applied a high sensitivity gamma-ray imaging system to image uptake and transport of radioactive 65 Zn in whole-plant assays of Arabidopsis thaliana and the Zn hyperaccumulator Arabidopsis halleri. We show that our system can be used to quantitatively image and measure uptake and root-to-shoot translocation dynamics of zinc in real time. In the metal hyperaccumulator Arabidopsis halleri, 65 Zn uptake and transport from its growth media to the shoot occurs rapidly and on time scales similar to those reported in rice. In transgenic A. halleri plants in which expression of the zinc transporter gene HMA4 is suppressed by RNAi, 65 Zn uptake is completely abolished.

Original languageEnglish (US)
Article numbere00131
JournalPlant Direct
Volume3
Issue number4
DOIs
StatePublished - Apr 1 2019

Fingerprint

Gamma Rays
Heavy Metals
Arabidopsis
Gamma rays
gamma radiation
heavy metals
Arabidopsis thaliana
image analysis
heavy metal
uptake mechanisms
Imaging techniques
Zinc
zinc
hyperaccumulators
Poisons
Toxic Plants
Imaging systems
shoot
Assays
Growth

Keywords

  • Arabidopsis halleri
  • metal hyperaccumulation
  • metal transport
  • metal uptake
  • nuclear imaging
  • single photon emission computed tomography
  • zinc

ASJC Scopus subject areas

  • Plant Science
  • Ecology
  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)

Cite this

Real-time whole-plant dynamics of heavy metal transport in Arabidopsis halleri and Arabidopsis thaliana by gamma-ray imaging. / Kajala, Kaisa; Walker, Katherine L.; Mitchell, Gregory; Krämer, Ute; Cherry, Simon R; Brady, Siobhan M.

In: Plant Direct, Vol. 3, No. 4, e00131, 01.04.2019.

Research output: Contribution to journalArticle

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