Real-time PCR and typing of Clostridium difficile isolates colonizing mare-foal pairs

K G Magdesian, C. M. Leutenegger

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Clostridium difficile infection can occur in the dams of sick foals, but it is unknown if mares and foals share the same isolates. In this study, C. difficile isolates from fecal samples of 11 mares paired with 11 foals were genotyped by arbitrarily primed PCR; two mares and three foals in five mare-foal pairs had diarrhea. Fecal immunoassays were utilized to detect C. difficile common antigen and toxin A. Quantitative real-time PCR (qPCR) systems were developed to detect genes for toxins A and B, as well as for binary toxin B. Sequences of all toxins were present in all isolates, although only one horse was positive for toxin A on fecal immunoassay. Identical strains of C. difficile were present in 4/11 (36.4%) mare-foal pairs. Mare-foal pairs can harbor C. difficile subclinically and are potential reservoirs for colonization of each other.

Original languageEnglish (US)
Pages (from-to)119-123
Number of pages5
JournalVeterinary Journal
Volume190
Issue number1
DOIs
StatePublished - Oct 2011

Fingerprint

Clostridium difficile
foals
mares
Real-Time Polymerase Chain Reaction
quantitative polymerase chain reaction
toxins
Immunoassay
immunoassays
Clostridium Infections
Horses
Diarrhea
Antigens
Polymerase Chain Reaction
diarrhea
antigens
horses
Genes
infection

Keywords

  • Clostridium difficile
  • Foals
  • Genotyping
  • Horses
  • Toxins

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Real-time PCR and typing of Clostridium difficile isolates colonizing mare-foal pairs. / Magdesian, K G; Leutenegger, C. M.

In: Veterinary Journal, Vol. 190, No. 1, 10.2011, p. 119-123.

Research output: Contribution to journalArticle

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