Real-time monitoring of flavivirus induced cytopathogenesis using cell electric impedance technology

Ying Fang, Peifang Ye, Xiaobo Wang, Xiao Xu, William Reisen

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

A real-time cell analysis (RTCA) system based on cell-substrate electric impedance technology was used to monitor cytopathic effects (CPE) in Vero cell cultures infected with West Nile virus (WNV) and St. Louis encephalitis virus (SLEV) at infectious doses ranging from 101 to 106 plaque forming units (PFU) of virus. A kinetic parameter characterizing virus-induced CPE, CIT50 or the time to 50% decrease in cell impedance, was inversely proportional to virus infectious dose. In WNV-infected cells, the onset and rate of CPE was earlier and faster than in SLEV-infected cells, which was consistent with viral cytolytic activity. A mathematical model simulating impedance-based CPE kinetic curves indicated that the replication rate of WNV was about 3 times faster than SLEV. The RTCA system also was used for quantifying the level of cell protection by specific neutralizing antibodies against WNV and SLEV. The onset of WNV or SLEV-induced CPE was delayed in the presence of specific anti-sera, and this delay in the CIT50 was well correlated with the titer of the neutralizing antibody as measured independently by plaque reduction neutralization tests (PRNT). The RTCA system provided a high throughput and quantitative method for real-time monitoring viral growth in cell culture and its inhibition by neutralizing antibodies.

Original languageEnglish (US)
Pages (from-to)251-258
Number of pages8
JournalJournal of Virological Methods
Volume173
Issue number2
DOIs
StatePublished - May 1 2011

Fingerprint

Flavivirus
St. Louis Encephalitis Viruses
Electric Impedance
West Nile virus
Technology
Neutralizing Antibodies
Viruses
Cell Culture Techniques
Neutralization Tests
Vero Cells
Cytoprotection
Theoretical Models
Growth
Serum

Keywords

  • Impedance
  • Neutralization assay
  • Real-time cell analysis system
  • St. Louis encephalitis virus
  • Viral cytopathogenesis
  • West Nile virus

ASJC Scopus subject areas

  • Virology

Cite this

Real-time monitoring of flavivirus induced cytopathogenesis using cell electric impedance technology. / Fang, Ying; Ye, Peifang; Wang, Xiaobo; Xu, Xiao; Reisen, William.

In: Journal of Virological Methods, Vol. 173, No. 2, 01.05.2011, p. 251-258.

Research output: Contribution to journalArticle

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